Structural diversity and functional implications of the eukaryotic TDP gene family

Hurng Yi Wang, I. Fan Wang, Jayaramakrishnan Bose, C. K.James Shen

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216 引文 斯高帕斯(Scopus)

摘要

TDP-43 is an RNA-binding protein that functions in mammalian cells in transcriptional repression and exon skipping. The gene encoding TDP-43 (HGMW-approved gene symbol TARDBP) is conserved in human, mouse, Drosophila melanogaster, and Caenorhabditis elegans. Sequence comparison of the coding regions of the TDP genes among the four taxa reveals an extraordinarily low rate of sequence divergence, suggesting that the TDP genes carry out essential functions in these organisms. With DNA transfection assay, we have established the importance of the glycine-rich domain for the exon-skipping activity of TDP-43. Both human and mouse TDP genes belong to a gene family that also consists of a number of processed pseudogenes. Interestingly, combined database analysis and cDNA cloning have demonstrated that the primary transcript of the mammalian TDP genes undergoes alternative splicing to generate 11 mRNAs, including the one encoding TDP-43. Eight of the 11 splicing events involved the use of four each of the 5′-donor and 3′-acceptor sites, all of which reside within the last exon of the TDP-43 mRNA. The existence of multispliced isoforms of TDP-encoded proteins provides further support for the functional complexity of the eukaryotic TDP genes.

原文英語
頁(從 - 到)130-139
頁數10
期刊Genomics
83
發行號1
DOIs
出版狀態已發佈 - 一月 2004
對外發佈Yes

ASJC Scopus subject areas

  • Genetics

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