Structural basis on the dityrosyl-diiron radical cluster and the functional differences of human ribonucleotide reductase small subunits hp53R2 and hRRM2

Bingsen Zhou, Leila Su, Yate Ching Yuan, Frank Un, Norby Wang, Madhukar Patel, Bixin Xi, Shuya Hu, Yun Yen

研究成果: 雜誌貢獻文章

8 引文 斯高帕斯(Scopus)

摘要

Ribonucleotide reductase (RNR) is an enzyme for the de novo conversion of ribonucleotides to deoxyribonucleotides. The two human RNR small subunits hRRM2 and hp53R2 share 83% sequence homology but show distinct expression patterns and function. Structural analyses of the oxidized form of hRRM2 and hp53R2 indicate that both proteins contain a conserved Gln127-hp53R2/Gln165-hRRM2 close to the dinuclear iron center and the essential tyrosine residue Tyr124-hp53R2/Tyr162- hRRM2 forms hydrogen bonds with the tyrosine and iron ligands, implying a critical role for the glutamine residue in assembling the dityrosyl-diiron radical cofactor. The present work also showed that Tyr221 in hRRM2, which is replaced by Phe183 in hp53R2, forms a hydrogen bond with Tyr162 to extend the hydrogen bond network from Gln165-hRRM2. Mutagenesis and spectroscopic experiments suggested that the tyrosine-to-phenylalanine switch at Phe183-hp53R2/Tyr221-hRRM2 could lead to differences in radical generation or enzymatic activity for hp53R2 and hRRM2. This study correlates the distinct catalytic mechanisms of the small subunits hp53R2 and hRRM2 with a hydrogen-bonding network and provides novel directions for designing and developing subunitspecific therapeutic agents for human RNR enzymes.

原文英語
頁(從 - 到)1669-1679
頁數11
期刊Molecular Cancer Therapeutics
9
發行號6
DOIs
出版狀態已發佈 - 六月 1 2010
對外發佈Yes

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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