Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma

Kwang Yu Chang, Chih Ta Huang, Tsung I. Hsu, Che Chia Hsu, Jr Jiun Liu, Cheng Keng Chuang, Jan Jong Hung, Wen Chang Chang, Kelvin K. Tsai, Jian Ying Chuang

研究成果: 雜誌貢獻文章

6 引文 斯高帕斯(Scopus)

摘要

It has been suggested that stress stimuli from the microenvironment maintain a subset of tumor cells with stem-like properties, including drug resistance. Here, we investigate whether Sp1, a stress-responsive factor, regulates stemness gene expression and if its inhibition sensitizes cancer cells to chemotherapy. Hydrogen peroxide- and serum deprivation-induced stresses were performed in glioblastoma (GBM) cells and patient-derived cells, and the effect of the Sp1 inhibitor mithramycin A (MA) on these stress-induced stem cells and temozolomide (TMZ)-resistant cells was evaluated. Sp1 and stemness genes were not commonly overexpressed in clinical GBM samples. However, their expression was highly induced by stress stimuli. Using MA, we demonstrated Sp1 as a critical stemness-related transcriptional factor protecting GBM cells against stress- and TMZ-induced death. Thus, Sp1 inhibition may prevent recurrence of malignant cells persisting after primary therapy.
原文英語
頁(從 - 到)14-19
頁數6
期刊Biochemical and Biophysical Research Communications
493
發行號1
DOIs
出版狀態已發佈 - 十一月 4 2017

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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