Sterol O-acyltransferase 2 contributes to the yolk cholesterol trafficking during zebrafish embryogenesis

Nai Yun Chang, Yen Ju Chan, Shih Torng Ding, Yen Hua Lee, Wei Chun Huangfu, I. Hsuan Liu

研究成果: 雜誌貢獻文章

4 引文 (Scopus)

摘要

To elucidate whether Sterol O-acyltransferase (Soat) mediates the absorption and transportation of yolk lipids to the developing embryo, zebrafish soat1 and soat2 were cloned and studied. In the adult zebrafish, soat1 was detected ubiquitously while soat2 mRNA was detected specifically in the liver, intestine, brain and testis. Whole mount in situ hybridization demonstrated that both soat1 and soat2 expressed in the yolk syncytial layer, hatching gland and developing cardiovascular as well as digestive systems, suggesting that Soats may play important roles in the lipid trafficking and utilization during embryonic development. The enzymatic activity of zebrafish Soat2 was confirmed by Oil Red O staining in the HEK293 cells overexpressing this gene, and could be quenched by Soat2 inhibitor Pyripyropene A (PPPA). The zebrafish embryos injected with PPPA or morpholino oligo against soat2 in the yolk showed significantly larger yolk when compared with wild-type embryos, especially at 72 hpf, indicating a slower rate of yolk consumption. Our result indicated that zebrafish Soat2 is catalytically active in synthesizing cholesteryl esters and contributes to the yolk cholesterol trafficking during zebrafish embryogenesis.
原文英語
文章編號e0167644
期刊PLoS One
11
發行號12
DOIs
出版狀態已發佈 - 十二月 1 2016

指紋

cholesterol acyltransferase
Zebrafish
Danio rerio
Embryonic Development
embryogenesis
Digestive system
Cholesterol
cholesterol
Sterol O-Acyltransferase
Lipids
Morpholinos
Cholesterol Esters
Liver
Brain
embryo (animal)
Embryonic Structures
Genes
Messenger RNA
Digestive System
cholesteryl esters

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

引用此文

Sterol O-acyltransferase 2 contributes to the yolk cholesterol trafficking during zebrafish embryogenesis. / Chang, Nai Yun; Chan, Yen Ju; Ding, Shih Torng; Lee, Yen Hua; Huangfu, Wei Chun; Liu, I. Hsuan.

於: PLoS One, 卷 11, 編號 12, e0167644, 01.12.2016.

研究成果: 雜誌貢獻文章

Chang, Nai Yun ; Chan, Yen Ju ; Ding, Shih Torng ; Lee, Yen Hua ; Huangfu, Wei Chun ; Liu, I. Hsuan. / Sterol O-acyltransferase 2 contributes to the yolk cholesterol trafficking during zebrafish embryogenesis. 於: PLoS One. 2016 ; 卷 11, 編號 12.
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