Stepped changes of monovalent ligand-binding force during ligand-induced clustering of integrin αIIBβ3

Chia Fen Hsieh, Bo Jui Chang, Chyi Huey Pai, Hsuan Yi Chen, Jin Wu Tsaia, Yung Hsiang Yi, Yi Ting Chiang, Da Wei Wang, Sien Chi, Long Hsu, Chi Hung Lin

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6 引文 斯高帕斯(Scopus)

摘要

Recent evidence demonstrated that conformational changes of the integrin during receptor activation affected its binding to extracellular matrix; however, experimental assessment of ligand-receptor binding following the initial molecular interaction has rarely been carried out at a single-molecule resolution. In the present study, laser tweezers were used to measure the binding force exerted by a live Chinese hamster ovary cell that expressed integrin αIIBβ3 (CHO α IIBβ3), to the bead carrier coated with the snake venom rhodostomin that served as an activated ligand for integrin αIIBβ3. A progressive increase of total binding force over time was noticed when the bead interacted with the CHO αIIBβ3 cell; such an increase was due mainly to the recruitment of more integrin molecules to the bead-cell interface. When the binding strength exerted by a single ligand-receptor pair was derived from the "polyvalent" measurements, surprisingly, a stepped decrease of the "monovalent binding force" was noted (from 4.15 to 2.54 piconewtons (pN)); such decrease appeared to occur during the ligand-induced integrin clustering process. On the other hand, the mutant rhodostomin defective in clustering integrins exhibited only one (1.81 pN) unit binding strength.

原文英語
頁(從 - 到)25466-25474
頁數9
期刊Journal of Biological Chemistry
281
發行號35
DOIs
出版狀態已發佈 - 9月 1 2006
對外發佈

ASJC Scopus subject areas

  • 生物化學

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