摘要
Purpose: The present study aims at investigating the involvement of several genes in the cell cycle distribution and apoptosis in U937 cells, a cell line lacking functional p53 protein, after combined treatment with staurosporine and irradiation. Materials and methods: Using a DNA fragmentation assay, flow cytometry and western blot analysis, the molecular basis for the effects of staurosporine in combination with the irradiation of leukemia cells was investigated. Results: Our results indicated that combined treatment led to an increased apoptotic cell death in U937 cells, which is correlated with the phosphorylation of the V-Jun sarcoma virus 17 oncogene homolog (c-JUN) NH 2-terminal kinase protein (JNK), the activation of caspases, the increase in B cell leukemia/lymphoma 2 (Bcl-2) associated X protein (Bax), the decrease in Bcl xL protein (Bcl-XL) levels, the loss of mitochondria membrane potential and the release of cytochrome c. Conclusions: Abrogation of the G2 checkpoint should be an effective strategy against p53-deficient leukemia cells to irradiation-induced cell killing.
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 97-109 |
| 頁數 | 13 |
| 期刊 | International Journal of Radiation Biology |
| 卷 | 82 |
| 發行號 | 2 |
| DOIs | |
| 出版狀態 | 已發佈 - 二月 2006 |
指紋
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Radiology Nuclear Medicine and imaging
- Radiological and Ultrasound Technology
- Nuclear Energy and Engineering
- Radiation
引用此文
Staurosporine modulates radiosensitivity and radiation-induced apoptosis in U937 cells. / Guo, How Ran; Chen, Chia Hsin; Ho, Sheng Yow; Ho, Yuan Soon; Chen, Rong Jane; Wang, Ying Jan.
於: International Journal of Radiation Biology, 卷 82, 編號 2, 02.2006, p. 97-109.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Staurosporine modulates radiosensitivity and radiation-induced apoptosis in U937 cells
AU - Guo, How Ran
AU - Chen, Chia Hsin
AU - Ho, Sheng Yow
AU - Ho, Yuan Soon
AU - Chen, Rong Jane
AU - Wang, Ying Jan
PY - 2006/2
Y1 - 2006/2
N2 - Purpose: The present study aims at investigating the involvement of several genes in the cell cycle distribution and apoptosis in U937 cells, a cell line lacking functional p53 protein, after combined treatment with staurosporine and irradiation. Materials and methods: Using a DNA fragmentation assay, flow cytometry and western blot analysis, the molecular basis for the effects of staurosporine in combination with the irradiation of leukemia cells was investigated. Results: Our results indicated that combined treatment led to an increased apoptotic cell death in U937 cells, which is correlated with the phosphorylation of the V-Jun sarcoma virus 17 oncogene homolog (c-JUN) NH 2-terminal kinase protein (JNK), the activation of caspases, the increase in B cell leukemia/lymphoma 2 (Bcl-2) associated X protein (Bax), the decrease in Bcl xL protein (Bcl-XL) levels, the loss of mitochondria membrane potential and the release of cytochrome c. Conclusions: Abrogation of the G2 checkpoint should be an effective strategy against p53-deficient leukemia cells to irradiation-induced cell killing.
AB - Purpose: The present study aims at investigating the involvement of several genes in the cell cycle distribution and apoptosis in U937 cells, a cell line lacking functional p53 protein, after combined treatment with staurosporine and irradiation. Materials and methods: Using a DNA fragmentation assay, flow cytometry and western blot analysis, the molecular basis for the effects of staurosporine in combination with the irradiation of leukemia cells was investigated. Results: Our results indicated that combined treatment led to an increased apoptotic cell death in U937 cells, which is correlated with the phosphorylation of the V-Jun sarcoma virus 17 oncogene homolog (c-JUN) NH 2-terminal kinase protein (JNK), the activation of caspases, the increase in B cell leukemia/lymphoma 2 (Bcl-2) associated X protein (Bax), the decrease in Bcl xL protein (Bcl-XL) levels, the loss of mitochondria membrane potential and the release of cytochrome c. Conclusions: Abrogation of the G2 checkpoint should be an effective strategy against p53-deficient leukemia cells to irradiation-induced cell killing.
KW - Apoptosis
KW - Cell cycle checkpoints
KW - Chemical modifiers
KW - Leukaemias
KW - Radiotherapy
UR - http://www.scopus.com/inward/record.url?scp=33645166806&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645166806&partnerID=8YFLogxK
U2 - 10.1080/09553000600589149
DO - 10.1080/09553000600589149
M3 - Article
C2 - 16546908
AN - SCOPUS:33645166806
VL - 82
SP - 97
EP - 109
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
SN - 0955-3002
IS - 2
ER -