Static magnetic field increases survival rate of dental pulp stem cells during DMSO-free cryopreservation

Shu Li Lin, Wei-Jen Chang, Chun Yen Lin, Sung-Chih Hsien, Sheng-Yang Lee, Kang Hsin Fan, Che-Tong Lin, Haw-Ming Huang

研究成果: 雜誌貢獻文章同行評審

23 引文 斯高帕斯(Scopus)

摘要

Successful and efficient cryopreservation of living cells and organs is a key clinical application of regenerative medicine. Recently, magnetic cryopreservation has been reported for intact tooth banking and cryopreservation of dental tissue. The aim of this study was to assess the cryoprotective effects of static magnetic fields (SMFs) on human dental pulp stem cells (DPSCs) during cryopreservation. Human DPSCs isolated from extracted teeth were frozen with a 0.4-T or 0.8-T SMF and then stored at −196 °C for 24 h. During freezing, the cells were suspended in freezing media containing with 0, 3 or 10% DMSO. After thawing, the changes in survival rate of the DPSCs were determined by flow cytometry. To understand the possible cryoprotective mechanisms of the SMF, the membrane fluidity of SMF-exposed DPSCs was tested. The results showed that when the freezing medium was DMSO-free, the survival rates of the thawed DPSCs increased 2- or 2.5-fold when the cells were exposed to 0.4-T or 0.8-T SMFs, respectively (p 
原文英語
頁(從 - 到)302-308
頁數7
期刊Electromagnetic Biology and Medicine
34
發行號4
DOIs
出版狀態已發佈 - 2015

ASJC Scopus subject areas

  • 生物物理學
  • 醫藥(雜項)

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