Static magnetic field attenuates lipopolysaccharide-induced inflammation in pulp cells by affecting cell membrane stability

Sung Chih Hsieh, Jeng Ting Tsao, Wei Zhen Lew, Ya Hui Chan, Lin Wen Lee, Che Tong Lin, Yung Kai Huang, Haw Ming Huang

研究成果: 雜誌貢獻文章同行評審

4 引文 斯高帕斯(Scopus)

摘要

One of the causes of dental pulpitis is lipopolysaccharide-(LPS-) induced inflammatory response. Following pulp tissue inflammation, odontoblasts, dental pulp cells (DPCs), and dental pulp stem cells (DPSCs) will activate and repair damaged tissue to maintain homeostasis. However, when LPS infection is too serious, dental repair is impossible and disease may progress to irreversible pulpitis. Therefore, the aim of this study was to examine whether static magnetic field (SMF) can attenuate inflammatory response of dental pulp cells challenged with LPS. In methodology, dental pulp cells were isolated from extracted teeth. The population of DPSCs in the cultured DPCs was identified by phenotypes and multilineage differentiation. The effects of 0.4 T SMF on DPCs were observed through MTT assay and fluorescent anisotropy assay. Our results showed that the SMF exposure had no effect on surface markers or multilineage differentiation capability. However, SMF exposure increases cell viability by 15%. In addition, SMF increased cell membrane rigidity which is directly related to higher fluorescent anisotropy. In the LPS-challenged condition, DPCs treated with SMF demonstrated a higher tolerance to LPS-induced inflammatory response when compared to untreated controls. According to these results, we suggest that 0.4 T SMF attenuates LPS-induced inflammatory response to DPCs by changing cell membrane stability.
原文英語
文章編號492683
期刊Scientific World Journal
2015
DOIs
出版狀態已發佈 - 2015

ASJC Scopus subject areas

  • 生物化學、遺傳與分子生物學 (全部)
  • 環境科學 (全部)
  • 醫藥 (全部)

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