Sperm mitochondrial DNA depletion in men with asthenospermia

Shu Huei Kao; Hsiang Tai Chao; Hwan Wun Liu; Tien Lin Liao; Yau Huei Wei

doi:10.1016/j.fertnstert.2003.11.056

Sperm mitochondrial DNA depletion in men with asthenospermia

Shu Huei Kao, Hsiang Tai Chao, Hwan Wun Liu, Tien Lin Liao, Yau Huei Wei

研究成果: 雜誌貢獻 › 文章 › 同行評審

64 引文斯高帕斯（Scopus）

摘要

Objective To determine the content of sperm mitochondrial DNA (mtDNA) in patients with asthenospermia or with poor sperm motility. Design Analysis of the content of mtDNA as the ratio between the amount of mtDNA and nuclear DNA by using a new concurrent polymerase chain reaction. Setting University hospital infertility center. Patient(s) Eighty-six men who sought infertility therapy. Intervention(s) Moving characteristics of sperm were examined with a computer-assisted semen analyzer. Main outcome measure(s) Sperm samples were classified into two groups, one with asthenospermia and the other with normal moving characteristics. The content of mtDNA in sperm was determined by polymerase chain reaction. We analyzed the mitochondrial mass by MitoTracker Green staining and analyzed DNA content with propidium iodide staining by flow cytometry. Result(s) A decrease in sperm mtDNA content was detected in patients with asthenospermia or with poor sperm motility (

原文	英語
頁（從 - 到）	66-73
頁數	8
期刊	Fertility and Sterility
卷	82
發行號	1
DOIs	https://doi.org/10.1016/j.fertnstert.2003.11.056
出版狀態	已發佈 - 7月 2004

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title = "Sperm mitochondrial DNA depletion in men with asthenospermia",

abstract = "Objective To determine the content of sperm mitochondrial DNA (mtDNA) in patients with asthenospermia or with poor sperm motility. Design Analysis of the content of mtDNA as the ratio between the amount of mtDNA and nuclear DNA by using a new concurrent polymerase chain reaction. Setting University hospital infertility center. Patient(s) Eighty-six men who sought infertility therapy. Intervention(s) Moving characteristics of sperm were examined with a computer-assisted semen analyzer. Main outcome measure(s) Sperm samples were classified into two groups, one with asthenospermia and the other with normal moving characteristics. The content of mtDNA in sperm was determined by polymerase chain reaction. We analyzed the mitochondrial mass by MitoTracker Green staining and analyzed DNA content with propidium iodide staining by flow cytometry. Result(s) A decrease in sperm mtDNA content was detected in patients with asthenospermia or with poor sperm motility (",

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AU - Kao, Shu Huei

AU - Chao, Hsiang Tai

AU - Liu, Hwan Wun

AU - Liao, Tien Lin

AU - Wei, Yau Huei

PY - 2004/7

Y1 - 2004/7

N2 - Objective To determine the content of sperm mitochondrial DNA (mtDNA) in patients with asthenospermia or with poor sperm motility. Design Analysis of the content of mtDNA as the ratio between the amount of mtDNA and nuclear DNA by using a new concurrent polymerase chain reaction. Setting University hospital infertility center. Patient(s) Eighty-six men who sought infertility therapy. Intervention(s) Moving characteristics of sperm were examined with a computer-assisted semen analyzer. Main outcome measure(s) Sperm samples were classified into two groups, one with asthenospermia and the other with normal moving characteristics. The content of mtDNA in sperm was determined by polymerase chain reaction. We analyzed the mitochondrial mass by MitoTracker Green staining and analyzed DNA content with propidium iodide staining by flow cytometry. Result(s) A decrease in sperm mtDNA content was detected in patients with asthenospermia or with poor sperm motility (

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KW - Depletion

KW - infertility

KW - mitochondrial DNA

KW - motility

KW - sperm

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Sperm mitochondrial DNA depletion in men with asthenospermia

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