Specific interaction of the recombinant disintegrin-like domain of MDC- 15 (Metargidin, ADAM-15) with integrin αvβ3

Xi Ping Zhang, Tetsuji Kamata, Kenji Yokoyama, Wilma Puzon-McLaughlin, Yoshikazu Takada

研究成果: 雜誌貢獻文章同行評審

194 引文 斯高帕斯(Scopus)

摘要

MDC-15 (ADAM-15, metargidin), a membrane-anchored metalloprotease/disintegrin/cysteine-rich protein, is expressed on the surface of a wide range of cells and has an RGD tripeptide in its disintegrin-like domain. MDC-15 is potentially involved in cell-cell interactions through its interaction with integrins. We expressed a recombinant MDC-15 disintegrin-like domain as a fusion protein with glutathione S-transferase (designated D-15) in bacteria and examined its binding function to integrins using mammalian cells expressing different recombinant integrins. We found that D-15 specifically interacts with αvβ3 but not with the other integrins tested (α2β1, α3β1, α4β1, α5β1, α6β1, α6β4, αvβ1, αIIbβ3, and αLβ2). Mutation of the tripeptide RGD to SGA totally blocked binding of D-15 to αvβ3, suggesting that D-15- αvβ3 interaction is ROD-dependent. When the sequence RPTRGD is mutated to NWKRGD, D-15 is recognized by both αIIbβ3 and αvβ3, suggesting that the receptor binding specificity is mediated by the sequence flanking the RGD tripeptide, as in snake venom disintegrins. These results indicate that the disintegrin-like domain of MDC-15 functions as an adhesion molecule and may be involved n αvβ3-mediated cell-cell interactions.

原文英語
頁(從 - 到)7345-7350
頁數6
期刊Journal of Biological Chemistry
273
發行號13
DOIs
出版狀態已發佈 - 三月 27 1998
對外發佈

ASJC Scopus subject areas

  • 生物化學

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