Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells

Chung Ren Lin, Kuan Hung Chen, Chien Hui Yang, Jiin Tsuey Cheng, Shyr Ming Sheen-Chen, Chih Hsien Wu, Wei Dih Sy, Yi Shen Chen

研究成果: 雜誌貢獻文章同行評審

16 引文 斯高帕斯(Scopus)

摘要

Background: Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge. The aim of this study was to develop a method for gene transfer into rat primary dorsal root ganglion neurons using sonoporation. Methods: Dissociated cells from adult rat dorsal root ganglion (DRG) cells were sonicated for 1-8 s at 2.5-10 W to determine the optimal ultrasound duration and power for gene transfection and cell survival. Transfection efficiency was compared between sonoporation, liposome and lentiviral vector gene transfer techniques. Results: The optimum ultrasound intensity was 5 W for 2 s and yielded an efficiency of gene transfection of 31% and a survival rate of 35%. Conclusions: Sonoporation can be optimized to minimize cell death and yield a high percentage of transfected neurons and that this technique can be easily applied to primary cultures of rat dorsal root ganglion neurons.

原文英語
文章編號44
期刊Journal of Biomedical Science
17
發行號1
DOIs
出版狀態已發佈 - 一月 1 2010
對外發佈

ASJC Scopus subject areas

  • 內分泌學、糖尿病和代謝
  • 分子生物學
  • 臨床生物化學
  • 細胞生物學
  • 生物化學(醫學)
  • 藥學(醫學)

指紋

深入研究「Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells」主題。共同形成了獨特的指紋。

引用此