Single-molecule manipulation and detection platform for studying cancer cell chemotaxis

May Show Chen, Pei Wen Peng, Bing Chun Liou, Hsiao Chen Kuo, Keng Liang Ou, Tzu Sen Yang

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

Chemotaxis of cancer cells is an essential component of tumor dissemination. The chemotactic response is comprised of three separate steps, including chemosensing, polarization and locomotion. We present an innovative approach on chemotaxis assay to address cancer cell chemotaxis. We applied a high-resolution optical tweezers system to manipulate epidermal growth factor (EGF)-coated beads positioned close to the filopodia, to locally stimulate HT29 cells expressing the EGF receptor (EGFR). We demonstrated that membrane protrusion at the leading edge induced by an EGF chemotaxis occurred at about 30∼40 s. In addition, the present observation revealed that the locomotion of HT29 cell depended on whether the HT29 cell sensed the presence of the chemoattractant EGF. We anticipate the proposed approach based on optical tweezers, together with the platform at single-cell level, could be applied to build a quick screening method for detection and treatment evaluation of many types of cancer during chemotaxis.
原文英語
頁(從 - 到)259-265
頁數7
期刊Journal of Polymer Engineering
34
發行號3
DOIs
出版狀態已發佈 - 5月 1 2014

ASJC Scopus subject areas

  • 材料化學
  • 聚合物和塑料
  • 化學工程 (全部)

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