Single injection of naked plasmid encoding α-melanocyte-stimulating hormone protects against thioacetamide-induced acute liver failure in mice

Cheng Haung Wang, Bruno Jawan, Tsung Hsing Lee, Kuo Sheng Hung, Wen Ying Chou, Cheng Nann Lu, Jong Kang Liu, Yann Jang Chen

研究成果: 雜誌貢獻文章

27 引文 (Scopus)

摘要

Oxidative stress has been implicated in the propagation of acute liver injury. The aim of our study was to investigate whether gene transfer of α-melanocyte-stimulating hormone (α-MSH), a potent anti-inflammatory peptide, could prevent fulminant hepatic failure in mice. Acute liver damage was induced by intraperitoneal administration of thioacetamide. Hydrodynamics-based gene transfection with α-MSH expression plasmid via rapid tail vein injection was initiated 1 day prior to intoxication. The mortality in the α-MSH-treated mice was significantly lower compared to the vehicle group 3 days after injury. Liver histology significantly improved and TUNEL-positive hepatocytes decreased in the treated mice. The degradation of IκBα, endogenous inhibitor of nuclear factor κB, and upregulation of inducible nitric oxide synthase and tumor necrosis factor-α mRNA levels were prevented in the α-MSH-treated group, indicating decreased oxidative stress and inflammation. These results suggest α-MSH gene therapy might protect against acute hepatic necroinflammatory damage with further potential applications.
原文英語
頁(從 - 到)153-161
頁數9
期刊Biochemical and Biophysical Research Communications
322
發行號1
DOIs
出版狀態已發佈 - 九月 10 2004
對外發佈Yes

指紋

Thioacetamide
Melanocyte-Stimulating Hormones
Acute Liver Failure
Liver
Plasmids
Injections
Oxidative stress
Oxidative Stress
Gene transfer
Gene therapy
Histology
In Situ Nick-End Labeling
Wounds and Injuries
Corrosion inhibitors
Nitric Oxide Synthase Type II
Hydrodynamics
Genetic Therapy
Genes
Transfection
Tail

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

引用此文

Single injection of naked plasmid encoding α-melanocyte-stimulating hormone protects against thioacetamide-induced acute liver failure in mice. / Wang, Cheng Haung; Jawan, Bruno; Lee, Tsung Hsing; Hung, Kuo Sheng; Chou, Wen Ying; Lu, Cheng Nann; Liu, Jong Kang; Chen, Yann Jang.

於: Biochemical and Biophysical Research Communications, 卷 322, 編號 1, 10.09.2004, p. 153-161.

研究成果: 雜誌貢獻文章

Wang, Cheng Haung ; Jawan, Bruno ; Lee, Tsung Hsing ; Hung, Kuo Sheng ; Chou, Wen Ying ; Lu, Cheng Nann ; Liu, Jong Kang ; Chen, Yann Jang. / Single injection of naked plasmid encoding α-melanocyte-stimulating hormone protects against thioacetamide-induced acute liver failure in mice. 於: Biochemical and Biophysical Research Communications. 2004 ; 卷 322, 編號 1. 頁 153-161.
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abstract = "Oxidative stress has been implicated in the propagation of acute liver injury. The aim of our study was to investigate whether gene transfer of α-melanocyte-stimulating hormone (α-MSH), a potent anti-inflammatory peptide, could prevent fulminant hepatic failure in mice. Acute liver damage was induced by intraperitoneal administration of thioacetamide. Hydrodynamics-based gene transfection with α-MSH expression plasmid via rapid tail vein injection was initiated 1 day prior to intoxication. The mortality in the α-MSH-treated mice was significantly lower compared to the vehicle group 3 days after injury. Liver histology significantly improved and TUNEL-positive hepatocytes decreased in the treated mice. The degradation of IκBα, endogenous inhibitor of nuclear factor κB, and upregulation of inducible nitric oxide synthase and tumor necrosis factor-α mRNA levels were prevented in the α-MSH-treated group, indicating decreased oxidative stress and inflammation. These results suggest α-MSH gene therapy might protect against acute hepatic necroinflammatory damage with further potential applications.",
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AU - Hung, Kuo Sheng

AU - Chou, Wen Ying

AU - Lu, Cheng Nann

AU - Liu, Jong Kang

AU - Chen, Yann Jang

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N2 - Oxidative stress has been implicated in the propagation of acute liver injury. The aim of our study was to investigate whether gene transfer of α-melanocyte-stimulating hormone (α-MSH), a potent anti-inflammatory peptide, could prevent fulminant hepatic failure in mice. Acute liver damage was induced by intraperitoneal administration of thioacetamide. Hydrodynamics-based gene transfection with α-MSH expression plasmid via rapid tail vein injection was initiated 1 day prior to intoxication. The mortality in the α-MSH-treated mice was significantly lower compared to the vehicle group 3 days after injury. Liver histology significantly improved and TUNEL-positive hepatocytes decreased in the treated mice. The degradation of IκBα, endogenous inhibitor of nuclear factor κB, and upregulation of inducible nitric oxide synthase and tumor necrosis factor-α mRNA levels were prevented in the α-MSH-treated group, indicating decreased oxidative stress and inflammation. These results suggest α-MSH gene therapy might protect against acute hepatic necroinflammatory damage with further potential applications.

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