Oxidative stress has been implicated in the propagation of acute liver injury. The aim of our study was to investigate whether gene transfer of α-melanocyte-stimulating hormone (α-MSH), a potent anti-inflammatory peptide, could prevent fulminant hepatic failure in mice. Acute liver damage was induced by intraperitoneal administration of thioacetamide. Hydrodynamics-based gene transfection with α-MSH expression plasmid via rapid tail vein injection was initiated 1 day prior to intoxication. The mortality in the α-MSH-treated mice was significantly lower compared to the vehicle group 3 days after injury. Liver histology significantly improved and TUNEL-positive hepatocytes decreased in the treated mice. The degradation of IκBα, endogenous inhibitor of nuclear factor κB, and upregulation of inducible nitric oxide synthase and tumor necrosis factor-α mRNA levels were prevented in the α-MSH-treated group, indicating decreased oxidative stress and inflammation. These results suggest α-MSH gene therapy might protect against acute hepatic necroinflammatory damage with further potential applications.
|頁（從 - 到）||153-161|
|期刊||Biochemical and Biophysical Research Communications|
|出版狀態||已發佈 - 九月 10 2004|
ASJC Scopus subject areas
- Molecular Biology
Wang, C. H., Jawan, B., Lee, T. H., Hung, K. S., Chou, W. Y., Lu, C. N., Liu, J. K., & Chen, Y. J. (2004). Single injection of naked plasmid encoding α-melanocyte-stimulating hormone protects against thioacetamide-induced acute liver failure in mice. Biochemical and Biophysical Research Communications, 322(1), 153-161. https://doi.org/10.1016/j.bbrc.2004.07.091