Simultaneous measurement of urinary ketamine, norketamine, and dehydronorketamine by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry

Gong-Jhe Wu, Han Chan, Maw Rong Lee, Chung Yu Chen, Dar Yu Yang, Fu Chou Cheng

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

A liquid chromatography/atmospheric pressure ionization mass spectrometry has been developed for the determination of ketamine, norketamine, and dehydronorketamine in human urine. A separation of these analytes in urine samples without tedious pretreatment procedures has been achieved within 10 min. Linear calibration curves of these analytes with coefficients better than 0.998 have been obtained over a wide range from 12.5 to 200 ng/mL. The accuracy was between 2.1% and 7.2% with detection limits at levels of 0.02 ng/mL, 0.02 ng/mL and 0.93 ng/mL for ketamine, norketamine and dehydronorketamine, respectively. The results demonstrate the suitability of the liquid chromatography/atmospheric pressure ionization mass spectrometry approach to analyze trace ketamine, norketamine and dehydronorketamine in urine. Urinary ketamine and norketamine levels were relatively low at 4-24 h intervals and were difficult to assay in a normal laboratory. In the present study, the determination of urinary dehydronorketamine levels at 2-24 hours appears to have a great potential for use in Schedule III controlled drugs management.
原文英語
頁(從 - 到)351-355
頁數5
期刊Journal of the Chinese Chemical Society
54
發行號2
出版狀態已發佈 - 2007

ASJC Scopus subject areas

  • Chemistry(all)

指紋 深入研究「Simultaneous measurement of urinary ketamine, norketamine, and dehydronorketamine by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry」主題。共同形成了獨特的指紋。

引用此