Simultaneous imaging of temporal changes of NF-κB activity and viable tumor cells in Huh7/NF-κB-tk-luc2/rfp tumor-bearing mice

Wei Hsun Wang, I. Tsang Chiang, Yu Chang Liu, Fei Ting Hsu, Hong Wen Chen, Chuan Lin Chen, Yi Jang Lee, Wuu Jyh Lin, Jeng Jong Hwang

研究成果: 雜誌貢獻文章

3 引文 (Scopus)

摘要

Few studies have reported that the effect of sorafenib on advanced human hepatocellular carcinoma (HCC) is taking place via the inhibition of NF-κB signal transduction. Here we constructed a human HCC Huh7 stable clone with NF-κB-responsive element to drive dual reporter genes, herpes simplex virus thymidine kinase (tk) and firefly luciferase (luc2), and co-transfected with a third red fluorescent protein (rfp) gene, renamed as Huh7/NF-κB- tkluc2/ rfp cells, and combined with bioluminescent imaging (BLI) and red fluorescent protein imaging (RFPI) to monitor the effect of sorafenib on NF-κB activation and tumor inhibition. The results show that sorafenib could suppress the NF-κB-DNA binding activity, and the expression of downstream effector proteins. Notably, the relative photon fluxes obtained from RFPI and BLI, which represent the viable tumor cells and cells with NF-κB activation, decreased after sorafenib treatment by 50 to 65%, and 87.5 to >90%, respectively, suggesting that NF-κB activation is suppressed in viable HCC cells by sorafenib. Simultaneous molecular imaging of the temporal change of NF-κB activity and of viable cells in the same Huh7/NF-κB-tk-luc2/rfp tumors of the animal may reflect the real status of NF-κB activity and the viable tumor cells at the time of imaging.
原文英語
頁(從 - 到)339-350
頁數12
期刊In Vivo
27
發行號3
出版狀態已發佈 - 五月 2013
對外發佈Yes

指紋

Bearings (structural)
Thymidine Kinase
Tumors
Cells
Imaging techniques
Chemical activation
Hepatocellular Carcinoma
Neoplasms
Genes
B-Form DNA
Firefly Luciferases
Molecular imaging
Signal transduction
Molecular Imaging
Viruses
Simplexvirus
Reporter Genes
Photons
Animals
red fluorescent protein

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

引用此文

Wang, W. H., Chiang, I. T., Liu, Y. C., Hsu, F. T., Chen, H. W., Chen, C. L., ... Hwang, J. J. (2013). Simultaneous imaging of temporal changes of NF-κB activity and viable tumor cells in Huh7/NF-κB-tk-luc2/rfp tumor-bearing mice. In Vivo, 27(3), 339-350.

Simultaneous imaging of temporal changes of NF-κB activity and viable tumor cells in Huh7/NF-κB-tk-luc2/rfp tumor-bearing mice. / Wang, Wei Hsun; Chiang, I. Tsang; Liu, Yu Chang; Hsu, Fei Ting; Chen, Hong Wen; Chen, Chuan Lin; Lee, Yi Jang; Lin, Wuu Jyh; Hwang, Jeng Jong.

於: In Vivo, 卷 27, 編號 3, 05.2013, p. 339-350.

研究成果: 雜誌貢獻文章

Wang, WH, Chiang, IT, Liu, YC, Hsu, FT, Chen, HW, Chen, CL, Lee, YJ, Lin, WJ & Hwang, JJ 2013, 'Simultaneous imaging of temporal changes of NF-κB activity and viable tumor cells in Huh7/NF-κB-tk-luc2/rfp tumor-bearing mice', In Vivo, 卷 27, 編號 3, 頁 339-350.
Wang, Wei Hsun ; Chiang, I. Tsang ; Liu, Yu Chang ; Hsu, Fei Ting ; Chen, Hong Wen ; Chen, Chuan Lin ; Lee, Yi Jang ; Lin, Wuu Jyh ; Hwang, Jeng Jong. / Simultaneous imaging of temporal changes of NF-κB activity and viable tumor cells in Huh7/NF-κB-tk-luc2/rfp tumor-bearing mice. 於: In Vivo. 2013 ; 卷 27, 編號 3. 頁 339-350.
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title = "Simultaneous imaging of temporal changes of NF-κB activity and viable tumor cells in Huh7/NF-κB-tk-luc2/rfp tumor-bearing mice",
abstract = "Few studies have reported that the effect of sorafenib on advanced human hepatocellular carcinoma (HCC) is taking place via the inhibition of NF-κB signal transduction. Here we constructed a human HCC Huh7 stable clone with NF-κB-responsive element to drive dual reporter genes, herpes simplex virus thymidine kinase (tk) and firefly luciferase (luc2), and co-transfected with a third red fluorescent protein (rfp) gene, renamed as Huh7/NF-κB- tkluc2/ rfp cells, and combined with bioluminescent imaging (BLI) and red fluorescent protein imaging (RFPI) to monitor the effect of sorafenib on NF-κB activation and tumor inhibition. The results show that sorafenib could suppress the NF-κB-DNA binding activity, and the expression of downstream effector proteins. Notably, the relative photon fluxes obtained from RFPI and BLI, which represent the viable tumor cells and cells with NF-κB activation, decreased after sorafenib treatment by 50 to 65{\%}, and 87.5 to >90{\%}, respectively, suggesting that NF-κB activation is suppressed in viable HCC cells by sorafenib. Simultaneous molecular imaging of the temporal change of NF-κB activity and of viable cells in the same Huh7/NF-κB-tk-luc2/rfp tumors of the animal may reflect the real status of NF-κB activity and the viable tumor cells at the time of imaging.",
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AU - Wang, Wei Hsun

AU - Chiang, I. Tsang

AU - Liu, Yu Chang

AU - Hsu, Fei Ting

AU - Chen, Hong Wen

AU - Chen, Chuan Lin

AU - Lee, Yi Jang

AU - Lin, Wuu Jyh

AU - Hwang, Jeng Jong

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AB - Few studies have reported that the effect of sorafenib on advanced human hepatocellular carcinoma (HCC) is taking place via the inhibition of NF-κB signal transduction. Here we constructed a human HCC Huh7 stable clone with NF-κB-responsive element to drive dual reporter genes, herpes simplex virus thymidine kinase (tk) and firefly luciferase (luc2), and co-transfected with a third red fluorescent protein (rfp) gene, renamed as Huh7/NF-κB- tkluc2/ rfp cells, and combined with bioluminescent imaging (BLI) and red fluorescent protein imaging (RFPI) to monitor the effect of sorafenib on NF-κB activation and tumor inhibition. The results show that sorafenib could suppress the NF-κB-DNA binding activity, and the expression of downstream effector proteins. Notably, the relative photon fluxes obtained from RFPI and BLI, which represent the viable tumor cells and cells with NF-κB activation, decreased after sorafenib treatment by 50 to 65%, and 87.5 to >90%, respectively, suggesting that NF-κB activation is suppressed in viable HCC cells by sorafenib. Simultaneous molecular imaging of the temporal change of NF-κB activity and of viable cells in the same Huh7/NF-κB-tk-luc2/rfp tumors of the animal may reflect the real status of NF-κB activity and the viable tumor cells at the time of imaging.

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