摘要
An accurate and reproducible method for the simultaneous determination of ampicillin (AMP), sulbactam (SUL), and cefoperazone (CFP) in pharmaceutical formulations by using HPLC with β-CD stationary phase was developed. It involved the use of the added tetraethylammonium acetate (TEAA) reagent, pH, and methanol as the significant parameters to find the optimum separation condition. A high resolution and selectivity of analytes was obtained by running the mobile phase in methanol-5 mM TEAA buffer = 35:65 (v/v, pH 4.5) at 280 nm. The mean recoveries ranged from 96.6 to 103.3% for AMP in the synthetic mixture, 97.6 to 103.0% for SUL, and 97.0 to 104.0% for CFP. The low LOD (<1.8 μg/mL) and low CV (<0.9%) assured that this method was sensitive and reproducible. The assay of analytes in commercial products exhibited that it was convenient and reproducible for routine analyses of these components in sterilized H2O, saline, or 5% dextrose injection solutions.
原文 | 英語 |
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頁(從 - 到) | 2407-2413 |
頁數 | 7 |
期刊 | Journal of Separation Science |
卷 | 30 |
發行號 | 15 |
DOIs | |
出版狀態 | 已發佈 - 10月 1 2007 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 分析化學
- 篩選和分離