Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells

Chin Lin Huang, I. Lun Hsiao, Ho Chen Lin, Chu Fang Wang, Yuh Jeen Huang, Chun Yu Chuang

研究成果: 雜誌貢獻文章

52 引文 (Scopus)

摘要

Silver nanoparticles (AgNPs) have antibacterial characteristics, and currently are applied in Ag-containing products. This study found neural cells can uptake 3-5. nm AgNPs, and investigated the potential effects of AgNPs on gene expression of inflammation and neurodegenerative disorder in murine brain ALT astrocytes, microglial BV-2 cells and neuron N2a cells. After AgNPs (5, 10, 12.5. μg/ml) exposure, these neural cells had obviously increased IL-1β secretion, and induced gene expression of C-X-C motif chemokine 13 (CXCL13), macrophage receptor with collagenous structure (MARCO) and glutathione synthetase (GSS) for inflammatory response and oxidative stress neutralization. Additionally, this study found amyloid-β (Aβ) plaques for pathological feature of Alzheimer's disease (AD) deposited in neural cells after AgNPs treatment. After AgNPs exposure, the gene expression of amyloid precursor protein (APP) was induced, and otherwise, neprilysin (NEP) and low-density lipoprotein receptor (LDLR) were reduced in neural cells as well as protein level. These results suggested AgNPs could alter gene and protein expressions of Aβ deposition potentially to induce AD progress in neural cells. It's necessary to take notice of AgNPs distribution in the environment.
原文英語
頁(從 - 到)253-263
頁數11
期刊Environmental Research
136
DOIs
出版狀態已發佈 - 一月 1 2015
對外發佈Yes

指紋

Silver
Gene expression
Nanoparticles
gene expression
brain
silver
Brain
Gene Expression
protein
Glutathione Synthase
Neprilysin
Oxidative stress
Amyloid beta-Protein Precursor
LDL Receptors
Macrophages
neutralization
Interleukin-1
Chemokines
Amyloid
secretion

ASJC Scopus subject areas

  • Biochemistry
  • Environmental Science(all)

引用此文

Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells. / Huang, Chin Lin; Hsiao, I. Lun; Lin, Ho Chen; Wang, Chu Fang; Huang, Yuh Jeen; Chuang, Chun Yu.

於: Environmental Research, 卷 136, 01.01.2015, p. 253-263.

研究成果: 雜誌貢獻文章

Huang, Chin Lin ; Hsiao, I. Lun ; Lin, Ho Chen ; Wang, Chu Fang ; Huang, Yuh Jeen ; Chuang, Chun Yu. / Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells. 於: Environmental Research. 2015 ; 卷 136. 頁 253-263.
@article{c6d0160a14004df18a8470ce5d0a8151,
title = "Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells",
abstract = "Silver nanoparticles (AgNPs) have antibacterial characteristics, and currently are applied in Ag-containing products. This study found neural cells can uptake 3-5. nm AgNPs, and investigated the potential effects of AgNPs on gene expression of inflammation and neurodegenerative disorder in murine brain ALT astrocytes, microglial BV-2 cells and neuron N2a cells. After AgNPs (5, 10, 12.5. μg/ml) exposure, these neural cells had obviously increased IL-1β secretion, and induced gene expression of C-X-C motif chemokine 13 (CXCL13), macrophage receptor with collagenous structure (MARCO) and glutathione synthetase (GSS) for inflammatory response and oxidative stress neutralization. Additionally, this study found amyloid-β (Aβ) plaques for pathological feature of Alzheimer's disease (AD) deposited in neural cells after AgNPs treatment. After AgNPs exposure, the gene expression of amyloid precursor protein (APP) was induced, and otherwise, neprilysin (NEP) and low-density lipoprotein receptor (LDLR) were reduced in neural cells as well as protein level. These results suggested AgNPs could alter gene and protein expressions of Aβ deposition potentially to induce AD progress in neural cells. It's necessary to take notice of AgNPs distribution in the environment.",
keywords = "Alzheimer 's disease, Gene expression, Inflammation, Neurodegenerative disorder, Silver nanoparticle",
author = "Huang, {Chin Lin} and Hsiao, {I. Lun} and Lin, {Ho Chen} and Wang, {Chu Fang} and Huang, {Yuh Jeen} and Chuang, {Chun Yu}",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.envres.2014.11.006",
language = "English",
volume = "136",
pages = "253--263",
journal = "Environmental Research",
issn = "0013-9351",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Silver nanoparticles affect on gene expression of inflammatory and neurodegenerative responses in mouse brain neural cells

AU - Huang, Chin Lin

AU - Hsiao, I. Lun

AU - Lin, Ho Chen

AU - Wang, Chu Fang

AU - Huang, Yuh Jeen

AU - Chuang, Chun Yu

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Silver nanoparticles (AgNPs) have antibacterial characteristics, and currently are applied in Ag-containing products. This study found neural cells can uptake 3-5. nm AgNPs, and investigated the potential effects of AgNPs on gene expression of inflammation and neurodegenerative disorder in murine brain ALT astrocytes, microglial BV-2 cells and neuron N2a cells. After AgNPs (5, 10, 12.5. μg/ml) exposure, these neural cells had obviously increased IL-1β secretion, and induced gene expression of C-X-C motif chemokine 13 (CXCL13), macrophage receptor with collagenous structure (MARCO) and glutathione synthetase (GSS) for inflammatory response and oxidative stress neutralization. Additionally, this study found amyloid-β (Aβ) plaques for pathological feature of Alzheimer's disease (AD) deposited in neural cells after AgNPs treatment. After AgNPs exposure, the gene expression of amyloid precursor protein (APP) was induced, and otherwise, neprilysin (NEP) and low-density lipoprotein receptor (LDLR) were reduced in neural cells as well as protein level. These results suggested AgNPs could alter gene and protein expressions of Aβ deposition potentially to induce AD progress in neural cells. It's necessary to take notice of AgNPs distribution in the environment.

AB - Silver nanoparticles (AgNPs) have antibacterial characteristics, and currently are applied in Ag-containing products. This study found neural cells can uptake 3-5. nm AgNPs, and investigated the potential effects of AgNPs on gene expression of inflammation and neurodegenerative disorder in murine brain ALT astrocytes, microglial BV-2 cells and neuron N2a cells. After AgNPs (5, 10, 12.5. μg/ml) exposure, these neural cells had obviously increased IL-1β secretion, and induced gene expression of C-X-C motif chemokine 13 (CXCL13), macrophage receptor with collagenous structure (MARCO) and glutathione synthetase (GSS) for inflammatory response and oxidative stress neutralization. Additionally, this study found amyloid-β (Aβ) plaques for pathological feature of Alzheimer's disease (AD) deposited in neural cells after AgNPs treatment. After AgNPs exposure, the gene expression of amyloid precursor protein (APP) was induced, and otherwise, neprilysin (NEP) and low-density lipoprotein receptor (LDLR) were reduced in neural cells as well as protein level. These results suggested AgNPs could alter gene and protein expressions of Aβ deposition potentially to induce AD progress in neural cells. It's necessary to take notice of AgNPs distribution in the environment.

KW - Alzheimer 's disease

KW - Gene expression

KW - Inflammation

KW - Neurodegenerative disorder

KW - Silver nanoparticle

UR - http://www.scopus.com/inward/record.url?scp=84911365647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84911365647&partnerID=8YFLogxK

U2 - 10.1016/j.envres.2014.11.006

DO - 10.1016/j.envres.2014.11.006

M3 - Article

VL - 136

SP - 253

EP - 263

JO - Environmental Research

JF - Environmental Research

SN - 0013-9351

ER -