Silk fibroin protein-based nonwoven mats incorporating baicalein Chinese herbal extract: Preparation, characterizations, and in vivo evaluation

Wing P. Chan, Kuan Chen Huang, Meng Yi Bai

研究成果: 雜誌貢獻文章同行評審

19 引文 斯高帕斯(Scopus)

摘要

In this study, we demonstrated a natural silk fibroin protein (SFP) that was blended with a Chinese herbal extract (baicalein, BAI) to obtain an effective combination for producing electrospun nonwoven mats with anti-inflammatory and antibacterial functions. A series of SFP-based electrospun nonwoven mats with additives of varying compositions were produced and investigated. Performance comparisons showed that the SFP/polyvinylpyrrolidone (PVP)/BAI nonwoven mat is the optimal one. In vitro, SFP/PVP/BAI nonwoven mat is effective in inhibiting the formation of nitrite in lipopolysaccharide (LPS)-induced nitrite formation in Raw 264.7 macrophages model and the growth of Staphylococcus aureus (S. aureus). Especially in the case of SFP/PVP/BAI nonwoven mat, Bai has been proved to reach their maximum amount of releases of approximately 64.8% within 24 h of contact with water-based environment as compared to the SFP/BAI nonwoven mat (only 30.1% of release within 24 h). For in vivo experiments, a 1.2 cm × 1.2 cm wound area was created on the back of mice and seeded with 1 × 107 CFU/mL of S. aureus to induce an infected wound model. The experimental results show significant acceleration of the wound closure process in mice treated with SFP/PVP/BAI nonwoven mat (4 days of reduction as compared to the untreated group), reduction in infiltration of neutrophils, nitrite formation, and inhibition of growth of wound bacteria. Histological images of the group treated with SFP/PVP/BAI nonwoven mat showed a compete repair of skin hierarchy, increasing production of collagen fibers, and enhancement of angiogenesis. This may bring a better recovery of skin appearance after treatment.
原文英語
頁(從 - 到)420-430
頁數11
期刊Journal of Biomedical Materials Research - Part B Applied Biomaterials
105
發行號2
DOIs
出版狀態已發佈 - 二月 1 2017

ASJC Scopus subject areas

  • 生物醫學工程
  • 生物材料

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