TY - JOUR
T1 - Significant elevation of plasma matrix metalloproteinase-9 level and its ratio to matrix metalloproteinase-2 in patients with pelvic inflammatory disease
AU - Wang, Po Hui
AU - Tsai, Hsiu Ting
AU - Tee, Yi Torng
AU - Lin, Long Yau
AU - Yang, Shun Fa
AU - Hsieh, Yih Shou
N1 - Funding Information:
Supported by Chung Shan Medical University, Taichung, Taiwan (CSMU 96-OM-B-028).
PY - 2009/11
Y1 - 2009/11
N2 - Objective: To detect the expression of plasma matrix metalloproteinase-2 (MMP-2) and MMP-9, and MMP-9:MMP-2 ratio in patients with pelvic inflammatory disease (PID). Design: A consecutive study with approximate 1:2 case-to-control ratio. Setting: University hospital. Patient(s): Forty-seven patients with PID and 80 healthy women. Intervention(s): Collected blood specimens of patients with PID before and after treatment. Main Outcome Measure(s): ELISA and gelatin zymography were used to measure the expression of plasma MMP-2 and MMP-9. Result(s): The level of plasma MMP-9 or MMP-9:MMP-2 ratio was elevated in patients with PID compared with healthy controls and decreased significantly after treatment. The activity of MMP-9, but not MMP-2, tended to be higher in 47 patients with PID before the treatment compared with that after the treatment. Pretreatment plasma MMP-9 or MMP-9:MMP-2 ratio was significantly correlated with white blood cell (WBC) and neutrophil counts. As prediction markers for PID, the sensitivities of MMP-9 and MMP-9:MMP-2 ratio were 76.6% and 76.6%, whereas the negative predictive values were 82.5% and 83.3%. Conclusion(s): Level of plasma MMP-9 and MMP-9:MMP-2 ratio may act as prediction markers for PID. In patients with PID, 80% have a plasma MMP-9 level higher than 115 ng/mL or a MMP-9:MMP-2 ratio higher than 2.15.
AB - Objective: To detect the expression of plasma matrix metalloproteinase-2 (MMP-2) and MMP-9, and MMP-9:MMP-2 ratio in patients with pelvic inflammatory disease (PID). Design: A consecutive study with approximate 1:2 case-to-control ratio. Setting: University hospital. Patient(s): Forty-seven patients with PID and 80 healthy women. Intervention(s): Collected blood specimens of patients with PID before and after treatment. Main Outcome Measure(s): ELISA and gelatin zymography were used to measure the expression of plasma MMP-2 and MMP-9. Result(s): The level of plasma MMP-9 or MMP-9:MMP-2 ratio was elevated in patients with PID compared with healthy controls and decreased significantly after treatment. The activity of MMP-9, but not MMP-2, tended to be higher in 47 patients with PID before the treatment compared with that after the treatment. Pretreatment plasma MMP-9 or MMP-9:MMP-2 ratio was significantly correlated with white blood cell (WBC) and neutrophil counts. As prediction markers for PID, the sensitivities of MMP-9 and MMP-9:MMP-2 ratio were 76.6% and 76.6%, whereas the negative predictive values were 82.5% and 83.3%. Conclusion(s): Level of plasma MMP-9 and MMP-9:MMP-2 ratio may act as prediction markers for PID. In patients with PID, 80% have a plasma MMP-9 level higher than 115 ng/mL or a MMP-9:MMP-2 ratio higher than 2.15.
KW - MMP-9:MMP-2 ratio
KW - Matrix metalloproteinase-9
KW - pelvic inflammatory disease
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U2 - 10.1016/j.fertnstert.2008.08.113
DO - 10.1016/j.fertnstert.2008.08.113
M3 - Article
C2 - 18937938
AN - SCOPUS:70350574842
VL - 92
SP - 1679
EP - 1684
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 5
ER -