Significant association between low mitochondrial DNA content in peripheral blood leukocytes and ischemic stroke

Li Ming Lien, Hung Yi Chiou, Hsu Ling Yeh, Shang Yen Chiu, Jiann Shing Jeng, Huey Juan Lin, Chaur Jong Hu, Fang I. Hsieh, Yau Huei Wei

研究成果: 雜誌貢獻文章

6 引文 (Scopus)

摘要

Background--Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. Methods and Results--A total of 350 patients with first-ever ischemic stroke and 350 healthy controls were recruited in this casecontrol study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real-time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8-hydroxy-20-deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8-hydroxy-20-deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. Conclusions--Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke.
原文英語
文章編號e006157
期刊Journal of the American Heart Association
6
發行號11
DOIs
出版狀態已發佈 - 十一月 1 2017

指紋

Mitochondrial DNA
Leukocytes
Stroke
Deoxyguanosine
Glutathione Disulfide
Oxidative Stress
Logistic Models
Glutathione
Real-Time Polymerase Chain Reaction
Atherosclerosis
Diabetes Mellitus
Smoking
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

引用此文

Significant association between low mitochondrial DNA content in peripheral blood leukocytes and ischemic stroke. / Lien, Li Ming; Chiou, Hung Yi; Yeh, Hsu Ling; Chiu, Shang Yen; Jeng, Jiann Shing; Lin, Huey Juan; Hu, Chaur Jong; Hsieh, Fang I.; Wei, Yau Huei.

於: Journal of the American Heart Association, 卷 6, 編號 11, e006157, 01.11.2017.

研究成果: 雜誌貢獻文章

@article{cca5b9fea71c46479df23fffabf9af24,
title = "Significant association between low mitochondrial DNA content in peripheral blood leukocytes and ischemic stroke",
abstract = "Background--Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. Methods and Results--A total of 350 patients with first-ever ischemic stroke and 350 healthy controls were recruited in this casecontrol study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real-time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8-hydroxy-20-deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8-hydroxy-20-deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. Conclusions--Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke.",
keywords = "Association study, Ischemic stroke, Mitochondrial DNA content",
author = "Lien, {Li Ming} and Chiou, {Hung Yi} and Yeh, {Hsu Ling} and Chiu, {Shang Yen} and Jeng, {Jiann Shing} and Lin, {Huey Juan} and Hu, {Chaur Jong} and Hsieh, {Fang I.} and Wei, {Yau Huei}",
year = "2017",
month = "11",
day = "1",
doi = "10.1161/JAHA.117.006157",
language = "English",
volume = "6",
journal = "Journal of the American Heart Association",
issn = "2047-9980",
publisher = "Wiley-Blackwell",
number = "11",

}

TY - JOUR

T1 - Significant association between low mitochondrial DNA content in peripheral blood leukocytes and ischemic stroke

AU - Lien, Li Ming

AU - Chiou, Hung Yi

AU - Yeh, Hsu Ling

AU - Chiu, Shang Yen

AU - Jeng, Jiann Shing

AU - Lin, Huey Juan

AU - Hu, Chaur Jong

AU - Hsieh, Fang I.

AU - Wei, Yau Huei

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Background--Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. Methods and Results--A total of 350 patients with first-ever ischemic stroke and 350 healthy controls were recruited in this casecontrol study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real-time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8-hydroxy-20-deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8-hydroxy-20-deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. Conclusions--Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke.

AB - Background--Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. Methods and Results--A total of 350 patients with first-ever ischemic stroke and 350 healthy controls were recruited in this casecontrol study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real-time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8-hydroxy-20-deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8-hydroxy-20-deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. Conclusions--Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke.

KW - Association study

KW - Ischemic stroke

KW - Mitochondrial DNA content

UR - http://www.scopus.com/inward/record.url?scp=85034790857&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034790857&partnerID=8YFLogxK

U2 - 10.1161/JAHA.117.006157

DO - 10.1161/JAHA.117.006157

M3 - Article

C2 - 29151031

AN - SCOPUS:85034790857

VL - 6

JO - Journal of the American Heart Association

JF - Journal of the American Heart Association

SN - 2047-9980

IS - 11

M1 - e006157

ER -