Signaling pathways for induction of platelet aggregation by sas tongue cancer cells - A mechanism of hematogenous metastasis

Mei Chi Chang, Chiu Po Chan, Yuan Soon Ho, Jang Jaer Lee, Po Shuen Lin, Bor Ru Lin, Ya Ling Huang, Liang Jiunn Hahn, Hung Wei Yeh, Ying Jan Wang, Jiiang Huei Jeng

研究成果: 雜誌貢獻文章同行評審

6 引文 斯高帕斯(Scopus)

摘要

Background: Tongue cancer metastasis is mainly through blood stream and possibly associated with tumor cell-induced platelet aggregation (TCIPA). Methods: Platelet aggregation was induced by different amounts of SAS tongue cancer cells with/without inhibitors and the latent period for induction of platelet aggregation was recorded. Gene expression was analyzed by reverse transcriptase-polymerase chain reaction. Results: SAS cells (4 × 10 4 to 1 × 10 6 cells/ml) induced platelet aggregation in a cell density-dependent manner. The latent period for induction of platelet aggregation reduced from 11.3 min (2 × 10 5 cells/ml) to 0.9 min (5 × 10 5 cells/ml). The extent of platelet aggregation increased from 39% to 76% by 2 × 10 5 and 5 × 10 5 SAS cells. Pre-treatment of SAS cells with aspirin showed little effect on its induction of platelet aggregation. SAS cells expressed tissue factor (TF) mRNA and the SAS cells-induced TCIPA was inhibited by TF neutralization antibody (5-20 μg/ml), heparin (5-10 U/ml), Hirudin fragment 54-65 (50 μg/ml) and D-Phenylalanyl-L-prolyl-L-arginine chloromethyl ketone. But areca nut (AN, a betel quid component known to generate reactive oxygen species (ROS)) extract showed little effect on TF expression in SAS cells. Pre-treatment with U73122 and 2-aminoethoxydiphenylborate inhibited SAS-induced TCIPA. Interestingly, catalase suppressed SAS cells-induced TCIPA, whereas AN extract enhanced this event. Conclusions: These results suggest that tongue cancer cells may induce TCIPA and enhance tumor metastasis. SAS-induced TCIPA is related to TF secretion, thrombin generation and associated with Phospholipase C-Inositol triphosphate signaling and ROS production. Betel quid chewing may potentially promote tongue cancer metastasis.

原文英語
頁(從 - 到)434-440
頁數7
期刊Journal of Oral Pathology and Medicine
38
發行號5
DOIs
出版狀態已發佈 - 五月 2009

ASJC Scopus subject areas

  • 癌症研究
  • 病理學與法醫學
  • 耳鼻咽喉科
  • 口腔外科
  • 牙周病

指紋

深入研究「Signaling pathways for induction of platelet aggregation by sas tongue cancer cells - A mechanism of hematogenous metastasis」主題。共同形成了獨特的指紋。

引用此