Shengmai San, a Chinese herbal medicine protects against rat heat stroke by reducing inflammatory cytokines and nitric oxide formation

Nien Lu Wang, Cheng Kuei Chang, Yann Lin Liou, Chia Li Lin, Mao Tsun Lin

研究成果: 雜誌貢獻文章

35 引文 (Scopus)

摘要

The aim of the present study was to ascertain whether the possible occurrence of overproduction of inducible nitric oxide synthase (iNOS)-dependent nitric oxide (NO) in the brain and inflammatory cytokines in the peripheral blood exhibited during heat stroke can be reduced by prior administration of Shengmai San, a Chinese herbal medicine. Aminoguanidine, an iNOS inhibitor, was evaluated at the same time as a reference (positive control). Urethane-anesthetized rats were exposed to heat stress (ambient temperature of 43°C) to induce heat stroke. Control rats were exposed to 24°C. Mean arterial pressure and cerebral blood flow after the onset of heat stroke were all significantly lower than in control rats. However, cerebral iNOS immunoreactivity and NO levels were all greater after the onset of heat stroke. The serum levels of interleukin-1β, interleukin-6, and tumor necrosis factor-α were all increased after the onset of heat stroke. Shengmai San (1.2g/ml per rat) or aminoguanidine (30 μmol/ml per rat) was administered orally, daily, and consecutively for 7 days before the initiation of heat stress; and this significantly attenuated the heat stress-induced arterial hypotension, cerebral ischemia, and increased levels of brain iNOS-dependent NO production and serum cytokines formation. Shengmai San shared with the aminoguanidine almost the same efficacy in reducing iNOS-dependent NO and cytokines overproduction during heat stroke. These results suggest that Shengmai San or aminoguanidine protects against heat stroke-induced arterial hypotension and cerebral ischemia by inhibition of iNOS-dependent NO overproduction in the brain and excessive accumulation of several inflammatory cytokines in the peripheral blood stream.
原文英語
頁(從 - 到)1-7
頁數7
期刊Journal of Pharmacological Sciences
98
發行號1
DOIs
出版狀態已發佈 - 五月 2005

指紋

Heat Stroke
Herbal Medicine
Nitric Oxide Synthase Type II
Nitric Oxide
Cytokines
Controlled Hypotension
Hot Temperature
Brain Ischemia
Cerebrovascular Circulation
Brain
Urethane
shengmai
Serum
Interleukin-1
Interleukin-6
Arterial Pressure
Tumor Necrosis Factor-alpha
Temperature
pimagedine

ASJC Scopus subject areas

  • Pharmacology

引用此文

Shengmai San, a Chinese herbal medicine protects against rat heat stroke by reducing inflammatory cytokines and nitric oxide formation. / Wang, Nien Lu; Chang, Cheng Kuei; Liou, Yann Lin; Lin, Chia Li; Lin, Mao Tsun.

於: Journal of Pharmacological Sciences, 卷 98, 編號 1, 05.2005, p. 1-7.

研究成果: 雜誌貢獻文章

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abstract = "The aim of the present study was to ascertain whether the possible occurrence of overproduction of inducible nitric oxide synthase (iNOS)-dependent nitric oxide (NO) in the brain and inflammatory cytokines in the peripheral blood exhibited during heat stroke can be reduced by prior administration of Shengmai San, a Chinese herbal medicine. Aminoguanidine, an iNOS inhibitor, was evaluated at the same time as a reference (positive control). Urethane-anesthetized rats were exposed to heat stress (ambient temperature of 43°C) to induce heat stroke. Control rats were exposed to 24°C. Mean arterial pressure and cerebral blood flow after the onset of heat stroke were all significantly lower than in control rats. However, cerebral iNOS immunoreactivity and NO levels were all greater after the onset of heat stroke. The serum levels of interleukin-1β, interleukin-6, and tumor necrosis factor-α were all increased after the onset of heat stroke. Shengmai San (1.2g/ml per rat) or aminoguanidine (30 μmol/ml per rat) was administered orally, daily, and consecutively for 7 days before the initiation of heat stress; and this significantly attenuated the heat stress-induced arterial hypotension, cerebral ischemia, and increased levels of brain iNOS-dependent NO production and serum cytokines formation. Shengmai San shared with the aminoguanidine almost the same efficacy in reducing iNOS-dependent NO and cytokines overproduction during heat stroke. These results suggest that Shengmai San or aminoguanidine protects against heat stroke-induced arterial hypotension and cerebral ischemia by inhibition of iNOS-dependent NO overproduction in the brain and excessive accumulation of several inflammatory cytokines in the peripheral blood stream.",
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