Shear stress induces synthetic-to-contractile phenotypic modulation in smooth muscle cells via peroxisome proliferator-activated receptor α/δ activations by prostacyclin released by sheared endothelial cells

Min Chien Tsai, Lihong Chen, Jing Zhou, Zhihui Tang, Tzu Fang Hsu, Ying Wang, Yu Tsung Shih, Hsin Hsin Peng, Nanping Wang, Youfei Guan, Shu Chien, Jeng Jiann Chiu

研究成果: 雜誌貢獻文章同行評審

78 引文 斯高帕斯(Scopus)

摘要

Rationale: Phenotypic modulation of smooth muscle cells (SMCs), which are located in close proximity to endothelial cells (ECs), is critical in regulating vascular function. The role of flow-induced shear stress in the modulation of SMC phenotype has not been well defined. Objective: The objective was to elucidate the role of shear stress on ECs in modulating SMC phenotype and its underlying mechanism. Methods and Results: Application of shear stress (12 dyn/cm2) to ECs cocultured with SMCs modulated SMC phenotype from synthetic to contractile state, with upregulation of contractile markers, downregulation of proinflammatory genes, and decreased percentage of cells in the synthetic phase. Treating SMCs with media from sheared ECs induced peroxisome proliferator-activated receptor (PPAR)-α, -δ, and -γ ligand binding activities; transfecting SMCs with specific small interfering (si)RNAs of PPAR-α and -δ, but not -γ, inhibited shear induction of contractile markers. ECs exposed to shear stress released prostacyclin (PGI2). Transfecting ECs with PGI2 synthase-specific siRNA inhibited shear-induced activation of PPAR-α/δ, upregulation of contractile markers, downregulation of proinflammatory genes, and decrease in percentage of SMCs in synthetic phase. Mice with PPAR-α deficiency (compared with control littermates) showed altered SMC phenotype oward a synthetic state, with increased arterial contractility in response to angiotensin II. Conclusions: These results indicate that laminar shear stress induces synthetic-to-contractile phenotypic modulation in SMCs through the activation of PPAR-α/δ by the EC-released PGI2. Our findings provide insights into the mechanisms underlying the EC-SMC interplays and the protective homeostatic function of laminar shear stress in modulating SMC phenotype.

原文英語
頁(從 - 到)471-480
頁數10
期刊Circulation Research
105
發行號5
DOIs
出版狀態已發佈 - 8月 28 2009
對外發佈

ASJC Scopus subject areas

  • 生理學
  • 心臟病學與心血管醫學

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