SEPT12-Microtubule complexes are required for sperm head and tail formation

Pao Lin Kuo, Han Sun Chiang, Ya Yun Wang, Yung Che Kuo, Mei Feng Chen, I. Shing Yu, Yen Ni Teng, Shu Wha Lin, Ying Hung Lin

研究成果: 雜誌貢獻文章

13 引文 斯高帕斯(Scopus)

摘要

The septin gene belongs to a highly conserved family of polymerizing GTP-binding cytoskeletal proteins. SEPTs perform cytoskeletal remodeling, cell polarity, mitosis, and vesicle trafficking by interacting with various cytoskeletons. Our previous studies have indicated that SEPTIN12+/+/+/- chimeras with a SEPTIN12 mutant allele were infertile. Spermatozoa from the vas deferens of chimeric mice indicated an abnormal sperm morphology, decreased sperm count, and immotile sperm. Mutations and genetic variants of SEPTIN12 in infertility cases also caused oligozoospermia and teratozoospermia. We suggest that a loss of SEPT12 affects the biological function of microtublin functions and causes spermiogenesis defects. In the cell model, SEPT12 interacts with α- and β-tubulins by co-immunoprecipitation (co-IP). To determine the precise localization and interactions between SEPT12 and α- and β-tubulins in vivo, we created SEPTIN12-transgene mice. We demonstrate how SEPT12 interacts and co-localizes with α- and β-tubulins during spermiogenesis in these mice. By using shRNA, the loss of SEPT12 transcripts disrupts α- and β-tubulin organization. In addition, losing or decreasing SEPT12 disturbs the morphogenesis of sperm heads and the elongation of sperm tails, the steps of which are coordinated and constructed by α- and β-tubulins, in SEPTIN12+/+/+/- chimeras. In this study, we discovered that the SEPTIN12-microtubule complexes are critical for sperm formation during spermiogenesis.
原文英語
頁(從 - 到)22102-22116
頁數15
期刊International Journal of Molecular Sciences
14
發行號11
DOIs
出版狀態已發佈 - 十一月 7 2013
對外發佈Yes

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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    Kuo, P. L., Chiang, H. S., Wang, Y. Y., Kuo, Y. C., Chen, M. F., Yu, I. S., Teng, Y. N., Lin, S. W., & Lin, Y. H. (2013). SEPT12-Microtubule complexes are required for sperm head and tail formation. International Journal of Molecular Sciences, 14(11), 22102-22116. https://doi.org/10.3390/ijms141122102