Semicarbazide-sensitive amine oxidase inhibitory and hydroxyl radical scavenging activities of aspartic acid β-hydroxamate and glutamic acid γ-monohydroxamate

Yuh Hwa Liu, Yin Shiou Lin, Tai Lin Lee, Wen Chi Hou

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

It was reported that semicarbazide-sensitive amine oxidase (SSAO) in plasma was elevated in diabetes mellitus and heart failure. SSAO catalyzed the oxidative deamination of various amines to produce hydrogen peroxide which is one possible source of oxidative stresses. In this research, two amino acid hydroxamates, namely aspartic acid β-hydroxamate (AAH) and glutamic acid γ-monohydroxamate (GAH), showed dose-dependently inhibitory against SSAO from bovine plasma. The IC50 of AAH and GAH was 0.7 mM and 0.023 mM, respectively, in the comparisons with 0.035 mM of semicarbazide (positive controls). The AAH showed mixed noncompetitive inhibition against bovine SSAO in the respect to benzylamine (substrate) and benzylamine-SSAO (substrate-enzyme complex). It was found that the V'max value was reduced and K'm value was increased in the presence of AAH (0.4 mM). Using electron spin resonance to investigate the hydroxyl radical scavenging activities, AAH and GAH showed effectively scavenging activities and IC50 was 0.66 mM and 1.21 mM, respectively.

原文英語
頁(從 - 到)301-306
頁數6
期刊Botanical Studies
53
發行號3
出版狀態已發佈 - 七月 2012

ASJC Scopus subject areas

  • 植物科學

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