Selective separation of virus proteins and double-stranded RNAs by SDS-KCl precipitation

Joseph K K Li, Todd Johnson, Yi Yuan Yang, Vicki Shore

研究成果: 雜誌貢獻文章

18 引文 (Scopus)

摘要

The total viral structural polypeptides and the double-stranded genomic RNAs of bluetongue virus can be selectively separated by a single SDS-KCl precipitation step. This simple, rapid and highly reproducible method enables greater than 95% recovery and purity of both viral proteins and dsRNAs within 30 min. The serotypic identity of the separated dsRNAs can be analyzed by SDS-PAGE electrophorogram immediately. After a single phenol/chloroform extraction, the dsRNA can also be used as hybridization probes, templates for molecular cloning and direct RNA sequencing. The SDS-KCl-precipitated viral proteins could be used readily for peptide mapping and as immunogens. Polyclonal and monoclonal antibodies raised against SDS-KCl-precipitated viral structural polypeptides were useful in Western immunoblots.

原文英語
頁(從 - 到)3-15
頁數13
期刊Journal of Virological Methods
26
發行號1
DOIs
出版狀態已發佈 - 1989
對外發佈Yes

指紋

Double-Stranded RNA
Viral Proteins
Bluetongue virus
Viruses
RNA Sequence Analysis
Peptides
Peptide Mapping
RNA Viruses
Molecular Cloning
Chloroform
Phenol
Polyacrylamide Gel Electrophoresis
Proteins
Western Blotting
Monoclonal Antibodies

ASJC Scopus subject areas

  • Virology

引用此文

Selective separation of virus proteins and double-stranded RNAs by SDS-KCl precipitation. / Li, Joseph K K; Johnson, Todd; Yang, Yi Yuan; Shore, Vicki.

於: Journal of Virological Methods, 卷 26, 編號 1, 1989, p. 3-15.

研究成果: 雜誌貢獻文章

Li, Joseph K K ; Johnson, Todd ; Yang, Yi Yuan ; Shore, Vicki. / Selective separation of virus proteins and double-stranded RNAs by SDS-KCl precipitation. 於: Journal of Virological Methods. 1989 ; 卷 26, 編號 1. 頁 3-15.
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