Selective inducible nitric oxide synthase suppression by new bracteanolides from Murdannia bracteata

Guei Jane Wang, Shih Ming Chen, Wei Chou Chen, Yu Min Chang, Zong-Huei Li

研究成果: 雜誌貢獻文章同行評審

27 引文 斯高帕斯(Scopus)

摘要

Murdannia bracteata has been used as a Taiwanese folk medicine for its anti-inflammatory properties. However, neither its active ingredients nor its anti-inflammatory actions are well defined. Nitric oxide (NO), overproduced by activated macrophages via inducible NO synthase (iNOS), is suggested to be a significant pathogenic factor in various inflammatory tissue injuries. In order to elucidate the anti-inflammatory actions of M. bracteata, the present study was designed to isolate its active constituents and examine its effects on iNOS in lipopolysaccharide (LPS)-activated macrophages. Two new hydroxybutenolides, bracteanolide A (1) and B (2), together with (+)-(R)-p-hydroxyphenyllactic acid (3) and isovitexin (4), were isolated and identified from M. bracteata by the NO production assay. All of the compounds inhibited NO production except 3. Their rank order of potency was 1 > 2 > 4. Among these, 1 significantly inhibited NO production, which is associated with its suppression on iNOS induction in a concentration-dependent manner, with an IC50 of 33.27 ± 0.86 μM. Nevertheless, isometric tension recordings in isolated endothelium-intact rat aorta revealed that 1-4 did not affect acetylcholine-induced endothelial NO-dependent relaxation, an index of endothelial NOS (eNOS) activity. The selective inhibition on iNOS provides a possible explanation for the anti-inflammatory use of M. bracteata.

原文英語
頁(從 - 到)221-227
頁數7
期刊Journal of Ethnopharmacology
112
發行號2
DOIs
出版狀態已發佈 - 六月 13 2007

ASJC Scopus subject areas

  • Pharmacology

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