Selective activation of specific PKC isoforms dictating the fate of CD14+ monocytes towards differentiation or apoptosis

研究成果: 雜誌貢獻文章

8 引文 (Scopus)

摘要

In this study, phorbol-12-myristate-13-acetate (PMA) at low concentrations (+ monocytes into monocyte-derived macrophages (MDMs) while PMA at high concentrations (>100-nM; H-PMA) causes the apoptosis of these cells. The pre-treatment with Go6976 (a PKC-α/β1 selective inhibitor), not anilinemonoindolylmaleimide [a PKC-β inhibitor (PKC-β inh.)], significantly (P-+ monocytes. On the other hand, either of the above two PKC inhibitors is capable of suppressing the H-PMA-induced apoptosis of CD14+ monocytes. However, only the inclusion of PKC-β inh., not Go6976, prevents the cells from serum deprivation-induced cell apoptosis. Although the membrane translocation of conventional PKC-α, β1, and β2 isoforms was observed in the H-PMA-treated CD14+ monocytes, only PKC-β2 exhibits a mitochondrial translocation activity among those PKCs responsive to H-PMA treatment. Moreover, the activation of DEVD-dependent caspases (DEVDase) was also detected in the H-PMA-treated CD14+ monocytes, indicating the involvement of a caspase-dependent signaling pathway in the H-PMA-induced cell apoptosis of CD14+ monocytes. Together with our previous findings that the selective activation of PKC-α or PKC-β1 induces the differentiation of CD14+ monocytes into MDMs or dendritic cells (MoDCs), respectively, the results in this study further demonstrate that PKC-β2 activation is responsible for relaying the apoptotic signal to intrinsic mitochondria-dependent caspase signaling cascades in the CD14+ monocytes. It is likely that the selective activation of specific PKC isoforms provides a new strategy to manipulate the differential cell fate commitment of multipotent CD14+ monocytes towards apoptosis or differentiation into MDMs, MoDCs, and other cell types.
原文英語
頁(從 - 到)122-131
頁數10
期刊Journal of Cellular Physiology
226
發行號1
DOIs
出版狀態已發佈 - 一月 2011

指紋

Monocytes
Protein Isoforms
Acetates
Chemical activation
Apoptosis
Macrophages
Caspases
Dendritic Cells
Mitochondria
phorbol-12-myristate
Membranes
Serum

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

引用此文

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title = "Selective activation of specific PKC isoforms dictating the fate of CD14+ monocytes towards differentiation or apoptosis",
abstract = "In this study, phorbol-12-myristate-13-acetate (PMA) at low concentrations (+ monocytes into monocyte-derived macrophages (MDMs) while PMA at high concentrations (>100-nM; H-PMA) causes the apoptosis of these cells. The pre-treatment with Go6976 (a PKC-α/β1 selective inhibitor), not anilinemonoindolylmaleimide [a PKC-β inhibitor (PKC-β inh.)], significantly (P-+ monocytes. On the other hand, either of the above two PKC inhibitors is capable of suppressing the H-PMA-induced apoptosis of CD14+ monocytes. However, only the inclusion of PKC-β inh., not Go6976, prevents the cells from serum deprivation-induced cell apoptosis. Although the membrane translocation of conventional PKC-α, β1, and β2 isoforms was observed in the H-PMA-treated CD14+ monocytes, only PKC-β2 exhibits a mitochondrial translocation activity among those PKCs responsive to H-PMA treatment. Moreover, the activation of DEVD-dependent caspases (DEVDase) was also detected in the H-PMA-treated CD14+ monocytes, indicating the involvement of a caspase-dependent signaling pathway in the H-PMA-induced cell apoptosis of CD14+ monocytes. Together with our previous findings that the selective activation of PKC-α or PKC-β1 induces the differentiation of CD14+ monocytes into MDMs or dendritic cells (MoDCs), respectively, the results in this study further demonstrate that PKC-β2 activation is responsible for relaying the apoptotic signal to intrinsic mitochondria-dependent caspase signaling cascades in the CD14+ monocytes. It is likely that the selective activation of specific PKC isoforms provides a new strategy to manipulate the differential cell fate commitment of multipotent CD14+ monocytes towards apoptosis or differentiation into MDMs, MoDCs, and other cell types.",
author = "Lin, {Yuan Feng} and Leu, {Sy Jye} and Huang, {Huei Mei} and Tsai, {Yu Hui}",
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T1 - Selective activation of specific PKC isoforms dictating the fate of CD14+ monocytes towards differentiation or apoptosis

AU - Lin, Yuan Feng

AU - Leu, Sy Jye

AU - Huang, Huei Mei

AU - Tsai, Yu Hui

PY - 2011/1

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N2 - In this study, phorbol-12-myristate-13-acetate (PMA) at low concentrations (+ monocytes into monocyte-derived macrophages (MDMs) while PMA at high concentrations (>100-nM; H-PMA) causes the apoptosis of these cells. The pre-treatment with Go6976 (a PKC-α/β1 selective inhibitor), not anilinemonoindolylmaleimide [a PKC-β inhibitor (PKC-β inh.)], significantly (P-+ monocytes. On the other hand, either of the above two PKC inhibitors is capable of suppressing the H-PMA-induced apoptosis of CD14+ monocytes. However, only the inclusion of PKC-β inh., not Go6976, prevents the cells from serum deprivation-induced cell apoptosis. Although the membrane translocation of conventional PKC-α, β1, and β2 isoforms was observed in the H-PMA-treated CD14+ monocytes, only PKC-β2 exhibits a mitochondrial translocation activity among those PKCs responsive to H-PMA treatment. Moreover, the activation of DEVD-dependent caspases (DEVDase) was also detected in the H-PMA-treated CD14+ monocytes, indicating the involvement of a caspase-dependent signaling pathway in the H-PMA-induced cell apoptosis of CD14+ monocytes. Together with our previous findings that the selective activation of PKC-α or PKC-β1 induces the differentiation of CD14+ monocytes into MDMs or dendritic cells (MoDCs), respectively, the results in this study further demonstrate that PKC-β2 activation is responsible for relaying the apoptotic signal to intrinsic mitochondria-dependent caspase signaling cascades in the CD14+ monocytes. It is likely that the selective activation of specific PKC isoforms provides a new strategy to manipulate the differential cell fate commitment of multipotent CD14+ monocytes towards apoptosis or differentiation into MDMs, MoDCs, and other cell types.

AB - In this study, phorbol-12-myristate-13-acetate (PMA) at low concentrations (+ monocytes into monocyte-derived macrophages (MDMs) while PMA at high concentrations (>100-nM; H-PMA) causes the apoptosis of these cells. The pre-treatment with Go6976 (a PKC-α/β1 selective inhibitor), not anilinemonoindolylmaleimide [a PKC-β inhibitor (PKC-β inh.)], significantly (P-+ monocytes. On the other hand, either of the above two PKC inhibitors is capable of suppressing the H-PMA-induced apoptosis of CD14+ monocytes. However, only the inclusion of PKC-β inh., not Go6976, prevents the cells from serum deprivation-induced cell apoptosis. Although the membrane translocation of conventional PKC-α, β1, and β2 isoforms was observed in the H-PMA-treated CD14+ monocytes, only PKC-β2 exhibits a mitochondrial translocation activity among those PKCs responsive to H-PMA treatment. Moreover, the activation of DEVD-dependent caspases (DEVDase) was also detected in the H-PMA-treated CD14+ monocytes, indicating the involvement of a caspase-dependent signaling pathway in the H-PMA-induced cell apoptosis of CD14+ monocytes. Together with our previous findings that the selective activation of PKC-α or PKC-β1 induces the differentiation of CD14+ monocytes into MDMs or dendritic cells (MoDCs), respectively, the results in this study further demonstrate that PKC-β2 activation is responsible for relaying the apoptotic signal to intrinsic mitochondria-dependent caspase signaling cascades in the CD14+ monocytes. It is likely that the selective activation of specific PKC isoforms provides a new strategy to manipulate the differential cell fate commitment of multipotent CD14+ monocytes towards apoptosis or differentiation into MDMs, MoDCs, and other cell types.

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