Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis

Sz Hsien Yu, Pei Ming Yang, Chih Wen Peng, Yi Chu Yu, Shu Jun Chiu

研究成果: 雜誌貢獻文章同行評審

28 引文 斯高帕斯(Scopus)

摘要

Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.
原文英語
頁(從 - 到)96-104
頁數9
期刊Cancer Letters
300
發行號1
DOIs
出版狀態已發佈 - 1月 1 2011
對外發佈

ASJC Scopus subject areas

  • 癌症研究
  • 腫瘤科

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