Screening mesenchymal stem cell attachment and differentiation on porous silicon gradients

Peng Yuan Wang, Lauren R. Clements, Helmut Thissen, Andrew Jane, Wei Bor Tsai, Nicolas H. Voelcker

研究成果: 雜誌貢獻文章同行評審

90 引文 斯高帕斯(Scopus)

摘要

The profound effects that nanoscale surface topography exerts on cell behavior are highly relevant to the development of advanced biomaterials and to advances in tissue engineering and regenerative medicine. Here, an asymmetric anodization procedure is used to produce n-type porous silicon (pSi) gradients with pore sizes ranging from tens to hundreds of nanometers in diameter and changes in the ridge nanoroughness from a few to tens nanometers. Rat mesenchymal stem cells (rMSCs) adhere poorly at the regions with small pore size but high ridge roughness. Cell adhesion is increased gradually towards the large pore size but low ridge roughness end of the pSi gradients. Surface topography influences cell differentiation, but not cell proliferation. Osteogenesis of rMSCs is enhanced by porous topography with a ridge roughness lower than 10 nm, while adipogenesis of rMSCs is enhanced on the entire pSi gradient compared with flat Si substrates. The results demonstrate that the gradient format allows in-depth screening of surface parameters that are important for the control of mammalian cell behavior, thereby advancing the development of new and improved biomaterials for orthopaedic and tissue engineering applications. An asymmetric anodization procedure is used to produce n-type porous silicon (pSi) gradients with pore sizes ranging from tens to hundreds of nanometers in diameter and changes in the ridge nanoroughness from a few to tens of nanometers. The results demonstrate that the gradient format allows in-depth screening of surface parameters that are important for the control of mammalian cell behavior, thereby advancing the development of new and improved biomaterials for orthopaedic and tissue engineering applications.
原文英語
頁(從 - 到)3414-3423
頁數10
期刊Advanced Functional Materials
22
發行號16
DOIs
出版狀態已發佈 - 八月 21 2012
對外發佈Yes

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

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