摘要
An ultra-high-performance liquid chromatography-quadrupole timeof-flight mass spectrometry (UHPLC-QTOF-MS) method for the screening and confirmation of 62 drugs of abuse and their metabolites in urine was developed in this study. The most commonly abused drugs, including amphetamines, opioids, cocaine, benzodiazepines (BZDs) and barbiturates, and many other new and emerging abused drugs, were selected as the analytes for this study. Urine samples were diluted 5-fold with deionized water before analysis. Using a superficially porous micro-particulate column and an acetic acid-based mobile phase, 54 basic and 8 acidic analytes could be detected within 15 and 12 min in positive and negative ionization modes, respectively. The MS collision energies for the 62 analytes were optimized, and their respective fragmentation patterns were constructed in the in-house library for confirmatory analysis. The coefficients of variation of the intra- and inter-day precision of the analyte responses all were
原文 | 英語 |
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文章編號 | bkt083 |
頁(從 - 到) | 642-651 |
頁數 | 10 |
期刊 | Journal of Analytical Toxicology |
卷 | 37 |
發行號 | 9 |
DOIs | |
出版狀態 | 已發佈 - 11月 2013 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 分析化學
- 毒理學
- 健康、毒理學和誘變
- 化學健康與安全
- 環境化學