Screening and confirmation of 62 drugs of abuse and metabolites in urine by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry

I. Lin Tsai, Te I. Weng, Yufeng J. Tseng, Happy Kuy Lok Tan, Hsiao Ju Sun, Ching Hua Kuo

研究成果: 雜誌貢獻文章同行評審

31 引文 斯高帕斯(Scopus)

摘要

An ultra-high-performance liquid chromatography-quadrupole timeof-flight mass spectrometry (UHPLC-QTOF-MS) method for the screening and confirmation of 62 drugs of abuse and their metabolites in urine was developed in this study. The most commonly abused drugs, including amphetamines, opioids, cocaine, benzodiazepines (BZDs) and barbiturates, and many other new and emerging abused drugs, were selected as the analytes for this study. Urine samples were diluted 5-fold with deionized water before analysis. Using a superficially porous micro-particulate column and an acetic acid-based mobile phase, 54 basic and 8 acidic analytes could be detected within 15 and 12 min in positive and negative ionization modes, respectively. The MS collision energies for the 62 analytes were optimized, and their respective fragmentation patterns were constructed in the in-house library for confirmatory analysis. The coefficients of variation of the intra- and inter-day precision of the analyte responses all were

原文英語
文章編號bkt083
頁(從 - 到)642-651
頁數10
期刊Journal of Analytical Toxicology
37
發行號9
DOIs
出版狀態已發佈 - 十一月 2013
對外發佈

ASJC Scopus subject areas

  • 分析化學
  • 毒理學
  • 健康、毒理學和誘變
  • 化學健康與安全
  • 環境化學

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