Scavenging effects of lotus seed extracts on reactive nitrogen species

Gow Chin Yen, Pin Der Duh, Hui Ju Su, Chi Tai Yeh, Chi Hao Wu

研究成果: 雜誌貢獻文章

43 引文 (Scopus)

摘要

The inhibitory effect of lotus (Nelumbo nicifera) seed extracts extracted with water (LSWE), ethyl acetate (LSEAE) and hexane (LSHE) on reactive nitrogen species, induced by DNA damage in macrophage RAW 264.7 cells, was investigated. The results showed that all extracts could inhibit nitric oxide accumulation in LPS-activated RAW 264.7 cells. The extracts in the range of 0.01-0.2 mg/ml showed a dose-dependent inhibitory effect on the accumulation of nitric oxide upon decomposition of sodium nitroprusside (SNP). The potency of inhibitory activity was in the order: LSEAE > LSWE > LSHE. The results of the comet assay indicated that the three extracts could inhibit DNA damage in macrophage RAW 264.7 cells induced by SNP. In addition, the three samples, at 0.2 mg/ml, showed 63%, 59%, and 38% inhibition of DNA damage in macrophage RAW 264.7 cells induced by peroxynitrite, respectively. All extracts tested were found to be potent peroxynitrite scavengers, capable of preventing the nitration of tyrosine. The data obtained suggest that lotus seed extracts might act as chemopreventers through reduction of excess amounts of nitric oxide.
原文英語
頁(從 - 到)596-602
頁數7
期刊Food Chemistry
94
發行號4
DOIs
出版狀態已發佈 - 三月 2006
對外發佈Yes

指紋

Reactive Nitrogen Species
Macrophages
seed extracts
Scavenging
hydrophobicity
Seed
Seeds
Nitric Oxide
Peroxynitrous Acid
Hexanes
Nitroprusside
DNA damage
DNA Damage
nitric oxide
macrophages
DNA
extracts
nitrogen
hexane
Nitration

ASJC Scopus subject areas

  • Food Science

引用此文

Scavenging effects of lotus seed extracts on reactive nitrogen species. / Yen, Gow Chin; Duh, Pin Der; Su, Hui Ju; Yeh, Chi Tai; Wu, Chi Hao.

於: Food Chemistry, 卷 94, 編號 4, 03.2006, p. 596-602.

研究成果: 雜誌貢獻文章

Yen, Gow Chin ; Duh, Pin Der ; Su, Hui Ju ; Yeh, Chi Tai ; Wu, Chi Hao. / Scavenging effects of lotus seed extracts on reactive nitrogen species. 於: Food Chemistry. 2006 ; 卷 94, 編號 4. 頁 596-602.
@article{255fd25124ae42e9963b34faa18e53be,
title = "Scavenging effects of lotus seed extracts on reactive nitrogen species",
abstract = "The inhibitory effect of lotus (Nelumbo nicifera) seed extracts extracted with water (LSWE), ethyl acetate (LSEAE) and hexane (LSHE) on reactive nitrogen species, induced by DNA damage in macrophage RAW 264.7 cells, was investigated. The results showed that all extracts could inhibit nitric oxide accumulation in LPS-activated RAW 264.7 cells. The extracts in the range of 0.01-0.2 mg/ml showed a dose-dependent inhibitory effect on the accumulation of nitric oxide upon decomposition of sodium nitroprusside (SNP). The potency of inhibitory activity was in the order: LSEAE > LSWE > LSHE. The results of the comet assay indicated that the three extracts could inhibit DNA damage in macrophage RAW 264.7 cells induced by SNP. In addition, the three samples, at 0.2 mg/ml, showed 63{\%}, 59{\%}, and 38{\%} inhibition of DNA damage in macrophage RAW 264.7 cells induced by peroxynitrite, respectively. All extracts tested were found to be potent peroxynitrite scavengers, capable of preventing the nitration of tyrosine. The data obtained suggest that lotus seed extracts might act as chemopreventers through reduction of excess amounts of nitric oxide.",
keywords = "Lotus seed, Macrophage RAW 264.7, Nitric oxide, Peroxynitrite, Single cell gel electrophoresis",
author = "Yen, {Gow Chin} and Duh, {Pin Der} and Su, {Hui Ju} and Yeh, {Chi Tai} and Wu, {Chi Hao}",
year = "2006",
month = "3",
doi = "10.1016/j.foodchem.2004.11.052",
language = "English",
volume = "94",
pages = "596--602",
journal = "Food Chemistry",
issn = "0308-8146",
publisher = "Elsevier Ltd",
number = "4",

}

TY - JOUR

T1 - Scavenging effects of lotus seed extracts on reactive nitrogen species

AU - Yen, Gow Chin

AU - Duh, Pin Der

AU - Su, Hui Ju

AU - Yeh, Chi Tai

AU - Wu, Chi Hao

PY - 2006/3

Y1 - 2006/3

N2 - The inhibitory effect of lotus (Nelumbo nicifera) seed extracts extracted with water (LSWE), ethyl acetate (LSEAE) and hexane (LSHE) on reactive nitrogen species, induced by DNA damage in macrophage RAW 264.7 cells, was investigated. The results showed that all extracts could inhibit nitric oxide accumulation in LPS-activated RAW 264.7 cells. The extracts in the range of 0.01-0.2 mg/ml showed a dose-dependent inhibitory effect on the accumulation of nitric oxide upon decomposition of sodium nitroprusside (SNP). The potency of inhibitory activity was in the order: LSEAE > LSWE > LSHE. The results of the comet assay indicated that the three extracts could inhibit DNA damage in macrophage RAW 264.7 cells induced by SNP. In addition, the three samples, at 0.2 mg/ml, showed 63%, 59%, and 38% inhibition of DNA damage in macrophage RAW 264.7 cells induced by peroxynitrite, respectively. All extracts tested were found to be potent peroxynitrite scavengers, capable of preventing the nitration of tyrosine. The data obtained suggest that lotus seed extracts might act as chemopreventers through reduction of excess amounts of nitric oxide.

AB - The inhibitory effect of lotus (Nelumbo nicifera) seed extracts extracted with water (LSWE), ethyl acetate (LSEAE) and hexane (LSHE) on reactive nitrogen species, induced by DNA damage in macrophage RAW 264.7 cells, was investigated. The results showed that all extracts could inhibit nitric oxide accumulation in LPS-activated RAW 264.7 cells. The extracts in the range of 0.01-0.2 mg/ml showed a dose-dependent inhibitory effect on the accumulation of nitric oxide upon decomposition of sodium nitroprusside (SNP). The potency of inhibitory activity was in the order: LSEAE > LSWE > LSHE. The results of the comet assay indicated that the three extracts could inhibit DNA damage in macrophage RAW 264.7 cells induced by SNP. In addition, the three samples, at 0.2 mg/ml, showed 63%, 59%, and 38% inhibition of DNA damage in macrophage RAW 264.7 cells induced by peroxynitrite, respectively. All extracts tested were found to be potent peroxynitrite scavengers, capable of preventing the nitration of tyrosine. The data obtained suggest that lotus seed extracts might act as chemopreventers through reduction of excess amounts of nitric oxide.

KW - Lotus seed

KW - Macrophage RAW 264.7

KW - Nitric oxide

KW - Peroxynitrite

KW - Single cell gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=24044461438&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24044461438&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2004.11.052

DO - 10.1016/j.foodchem.2004.11.052

M3 - Article

AN - SCOPUS:24044461438

VL - 94

SP - 596

EP - 602

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

IS - 4

ER -