Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinoma

Chung-Ji Liu, Jen-Hao Chen, Shih-Min Hsia, Chiu-Chu Liao, Hui-Wen Chang, Tzong-Ming Shieh, Yin-Hwa Shih

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

Background. The X-linked tumor suppressor gene LDOC1 is reported to be involved in oral cancer. The detection of biomarkers in salivary RNA is a non-invasive strategy for diagnosing many diseases. The aim of the present study was to investigate the potential of salivary LDOC1 as a biomarker of oral cancer. Methods. We determined the expression levels of LDOC1 in the saliva of oral squamous cell carcinoma (OSCC) subjects, and investigated its correlation with various clinicopathological characteristics. The expression levels of salivary LDOC1 were detected in 53 OSCC subjects and 43 healthy controls using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. We used Fisher's exact test to analyze the correlations between expression levels and clinicopathological characteristics. Results. Salivary LDOC1 was significantly upregulated in females with OSCC (p = 0.0072), and significantly downregulated in males with OSCC (p = 0.0206). Eighty-nine percent of male OSCC subjects who smoked expressed low levels of LDOC1. OSCC cell lines derived from male OSCC subjects expressed low levels of LDOC1. Conclusions. A high level of salivary LDOC1 expression is a biomarker of OSCC in females. A high percentage of male OSCC subjects who smoke express low levels of salivary LDOC1. A low level of salivary LDOC1 expression is a biomarker of OSCC in males.

原文英語
文章編號e6732
頁(從 - 到)e6732
期刊PeerJ
2019
發行號4
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

squamous cell carcinoma
Biomarkers
gender differences
Squamous Cell Carcinoma
mouth
biomarkers
Mouth Neoplasms
Epithelial Cells
tumor suppressor genes
Polymerase chain reaction
Transcription
smoke
saliva
Tumor Suppressor Genes
Saliva
Smoke
Reverse Transcription
Tumors
Healthy Volunteers
quantitative polymerase chain reaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)

引用此文

Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinoma. / Liu, Chung-Ji; Chen, Jen-Hao; Hsia, Shih-Min; Liao, Chiu-Chu; Chang, Hui-Wen; Shieh, Tzong-Ming; Shih, Yin-Hwa.

於: PeerJ, 卷 2019, 編號 4, e6732, 01.01.2019, p. e6732.

研究成果: 雜誌貢獻文章

Liu, Chung-Ji ; Chen, Jen-Hao ; Hsia, Shih-Min ; Liao, Chiu-Chu ; Chang, Hui-Wen ; Shieh, Tzong-Ming ; Shih, Yin-Hwa. / Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinoma. 於: PeerJ. 2019 ; 卷 2019, 編號 4. 頁 e6732.
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abstract = "Background. The X-linked tumor suppressor gene LDOC1 is reported to be involved in oral cancer. The detection of biomarkers in salivary RNA is a non-invasive strategy for diagnosing many diseases. The aim of the present study was to investigate the potential of salivary LDOC1 as a biomarker of oral cancer. Methods. We determined the expression levels of LDOC1 in the saliva of oral squamous cell carcinoma (OSCC) subjects, and investigated its correlation with various clinicopathological characteristics. The expression levels of salivary LDOC1 were detected in 53 OSCC subjects and 43 healthy controls using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. We used Fisher's exact test to analyze the correlations between expression levels and clinicopathological characteristics. Results. Salivary LDOC1 was significantly upregulated in females with OSCC (p = 0.0072), and significantly downregulated in males with OSCC (p = 0.0206). Eighty-nine percent of male OSCC subjects who smoked expressed low levels of LDOC1. OSCC cell lines derived from male OSCC subjects expressed low levels of LDOC1. Conclusions. A high level of salivary LDOC1 expression is a biomarker of OSCC in females. A high percentage of male OSCC subjects who smoke express low levels of salivary LDOC1. A low level of salivary LDOC1 expression is a biomarker of OSCC in males.",
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AU - Chang, Hui-Wen

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N2 - Background. The X-linked tumor suppressor gene LDOC1 is reported to be involved in oral cancer. The detection of biomarkers in salivary RNA is a non-invasive strategy for diagnosing many diseases. The aim of the present study was to investigate the potential of salivary LDOC1 as a biomarker of oral cancer. Methods. We determined the expression levels of LDOC1 in the saliva of oral squamous cell carcinoma (OSCC) subjects, and investigated its correlation with various clinicopathological characteristics. The expression levels of salivary LDOC1 were detected in 53 OSCC subjects and 43 healthy controls using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. We used Fisher's exact test to analyze the correlations between expression levels and clinicopathological characteristics. Results. Salivary LDOC1 was significantly upregulated in females with OSCC (p = 0.0072), and significantly downregulated in males with OSCC (p = 0.0206). Eighty-nine percent of male OSCC subjects who smoked expressed low levels of LDOC1. OSCC cell lines derived from male OSCC subjects expressed low levels of LDOC1. Conclusions. A high level of salivary LDOC1 expression is a biomarker of OSCC in females. A high percentage of male OSCC subjects who smoke express low levels of salivary LDOC1. A low level of salivary LDOC1 expression is a biomarker of OSCC in males.

AB - Background. The X-linked tumor suppressor gene LDOC1 is reported to be involved in oral cancer. The detection of biomarkers in salivary RNA is a non-invasive strategy for diagnosing many diseases. The aim of the present study was to investigate the potential of salivary LDOC1 as a biomarker of oral cancer. Methods. We determined the expression levels of LDOC1 in the saliva of oral squamous cell carcinoma (OSCC) subjects, and investigated its correlation with various clinicopathological characteristics. The expression levels of salivary LDOC1 were detected in 53 OSCC subjects and 43 healthy controls using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. We used Fisher's exact test to analyze the correlations between expression levels and clinicopathological characteristics. Results. Salivary LDOC1 was significantly upregulated in females with OSCC (p = 0.0072), and significantly downregulated in males with OSCC (p = 0.0206). Eighty-nine percent of male OSCC subjects who smoked expressed low levels of LDOC1. OSCC cell lines derived from male OSCC subjects expressed low levels of LDOC1. Conclusions. A high level of salivary LDOC1 expression is a biomarker of OSCC in females. A high percentage of male OSCC subjects who smoke express low levels of salivary LDOC1. A low level of salivary LDOC1 expression is a biomarker of OSCC in males.

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