Salicylic acid mediates alternative signal transduction pathways for pathogenesis-related acidic β-1,3-glucanase (protein N) induction in tobacco cell suspension culture

Hsien Jung Chen, Wen Chi Hou, Joseph Kuc', Yaw Huei Lin

研究成果: 雜誌貢獻文章

9 引文 (Scopus)

摘要

Salicylic acid (SA) plays a central role in plant acquired resistance and induces gene expression of a group of pathogenesis-related (PR) proteins. We have previously reported that tobacco cells display Ca2+-dependent and Ca2+-independent excretion modes of SA in suspension culture depending on the applied SA concentrations (Chen et al. 2001). In this study, same concentrations of SA (200 μmol/L and 20 μmol/L) were used to compare their effects on PR-N, an acidic β-1,3-glucanase, gene induction and mRNA accumulation in tobacco cell suspension culture. SA at both concentrations induced PR-N gene expression. The induction by 200 μmol/L SA was significantly inhibited by EGTA (a Ca2+ ion chelator) or reduced glutathione (an active oxygen species scavenger). However, these two compounds have little or no effect on PR-N gene induction by 20 μmol/L SA. Either calcium ionophore (A23187) or catalase inhibitor (3-amino-1,2,4-triazole; 3AT) activated PR-N gene expression. As observed for 200 μmol/L SA, the induction by A23187 and 3AT was also blocked by EGTA and reduced glutathione, respectively. A SA functional analog, 2,6-dichloroisonicotinic acid (INA), induced PR-N gene expression. However, EGTA and reduced glutathione had little or no effect on the induction by 20 μmol/L INA, same as observed for the induction by 20 μmol/L SA. Based on these data, we conclude that SA could mediate alternative signal transduction pathways leading to PR-N gene induction in tobacco cell suspension culture. SA concentration seems to play a key role in the activation of different components of signal transduction pathways. Active oxygen species elevation and external Ca2+ influx are components likely associated with 200 μmol/L SA activation mechanism. Another signal transduction pathway not associated with these two components, however, may be responsible for the effect of SA at 20 μmol/L.

原文英語
頁(從 - 到)331-337
頁數7
期刊Journal of Plant Physiology
159
發行號4
出版狀態已發佈 - 2002

指紋

Salicylic Acid
salicylic acid
cell suspension culture
Tobacco
signal transduction
Signal Transduction
Suspensions
tobacco
pathogenesis
Cell Culture Techniques
Proteins
proteins
gene induction
Egtazic Acid
calcium
Glutathione
Gene Expression
glutathione
gene expression
Calcimycin

ASJC Scopus subject areas

  • Plant Science

引用此文

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title = "Salicylic acid mediates alternative signal transduction pathways for pathogenesis-related acidic β-1,3-glucanase (protein N) induction in tobacco cell suspension culture",
abstract = "Salicylic acid (SA) plays a central role in plant acquired resistance and induces gene expression of a group of pathogenesis-related (PR) proteins. We have previously reported that tobacco cells display Ca2+-dependent and Ca2+-independent excretion modes of SA in suspension culture depending on the applied SA concentrations (Chen et al. 2001). In this study, same concentrations of SA (200 μmol/L and 20 μmol/L) were used to compare their effects on PR-N, an acidic β-1,3-glucanase, gene induction and mRNA accumulation in tobacco cell suspension culture. SA at both concentrations induced PR-N gene expression. The induction by 200 μmol/L SA was significantly inhibited by EGTA (a Ca2+ ion chelator) or reduced glutathione (an active oxygen species scavenger). However, these two compounds have little or no effect on PR-N gene induction by 20 μmol/L SA. Either calcium ionophore (A23187) or catalase inhibitor (3-amino-1,2,4-triazole; 3AT) activated PR-N gene expression. As observed for 200 μmol/L SA, the induction by A23187 and 3AT was also blocked by EGTA and reduced glutathione, respectively. A SA functional analog, 2,6-dichloroisonicotinic acid (INA), induced PR-N gene expression. However, EGTA and reduced glutathione had little or no effect on the induction by 20 μmol/L INA, same as observed for the induction by 20 μmol/L SA. Based on these data, we conclude that SA could mediate alternative signal transduction pathways leading to PR-N gene induction in tobacco cell suspension culture. SA concentration seems to play a key role in the activation of different components of signal transduction pathways. Active oxygen species elevation and external Ca2+ influx are components likely associated with 200 μmol/L SA activation mechanism. Another signal transduction pathway not associated with these two components, however, may be responsible for the effect of SA at 20 μmol/L.",
keywords = "Calcium, Glutathione, Pathogenesis-related protein, Salicyclic acid, Signal",
author = "Chen, {Hsien Jung} and Hou, {Wen Chi} and Joseph Kuc' and Lin, {Yaw Huei}",
year = "2002",
language = "English",
volume = "159",
pages = "331--337",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
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TY - JOUR

T1 - Salicylic acid mediates alternative signal transduction pathways for pathogenesis-related acidic β-1,3-glucanase (protein N) induction in tobacco cell suspension culture

AU - Chen, Hsien Jung

AU - Hou, Wen Chi

AU - Kuc', Joseph

AU - Lin, Yaw Huei

PY - 2002

Y1 - 2002

N2 - Salicylic acid (SA) plays a central role in plant acquired resistance and induces gene expression of a group of pathogenesis-related (PR) proteins. We have previously reported that tobacco cells display Ca2+-dependent and Ca2+-independent excretion modes of SA in suspension culture depending on the applied SA concentrations (Chen et al. 2001). In this study, same concentrations of SA (200 μmol/L and 20 μmol/L) were used to compare their effects on PR-N, an acidic β-1,3-glucanase, gene induction and mRNA accumulation in tobacco cell suspension culture. SA at both concentrations induced PR-N gene expression. The induction by 200 μmol/L SA was significantly inhibited by EGTA (a Ca2+ ion chelator) or reduced glutathione (an active oxygen species scavenger). However, these two compounds have little or no effect on PR-N gene induction by 20 μmol/L SA. Either calcium ionophore (A23187) or catalase inhibitor (3-amino-1,2,4-triazole; 3AT) activated PR-N gene expression. As observed for 200 μmol/L SA, the induction by A23187 and 3AT was also blocked by EGTA and reduced glutathione, respectively. A SA functional analog, 2,6-dichloroisonicotinic acid (INA), induced PR-N gene expression. However, EGTA and reduced glutathione had little or no effect on the induction by 20 μmol/L INA, same as observed for the induction by 20 μmol/L SA. Based on these data, we conclude that SA could mediate alternative signal transduction pathways leading to PR-N gene induction in tobacco cell suspension culture. SA concentration seems to play a key role in the activation of different components of signal transduction pathways. Active oxygen species elevation and external Ca2+ influx are components likely associated with 200 μmol/L SA activation mechanism. Another signal transduction pathway not associated with these two components, however, may be responsible for the effect of SA at 20 μmol/L.

AB - Salicylic acid (SA) plays a central role in plant acquired resistance and induces gene expression of a group of pathogenesis-related (PR) proteins. We have previously reported that tobacco cells display Ca2+-dependent and Ca2+-independent excretion modes of SA in suspension culture depending on the applied SA concentrations (Chen et al. 2001). In this study, same concentrations of SA (200 μmol/L and 20 μmol/L) were used to compare their effects on PR-N, an acidic β-1,3-glucanase, gene induction and mRNA accumulation in tobacco cell suspension culture. SA at both concentrations induced PR-N gene expression. The induction by 200 μmol/L SA was significantly inhibited by EGTA (a Ca2+ ion chelator) or reduced glutathione (an active oxygen species scavenger). However, these two compounds have little or no effect on PR-N gene induction by 20 μmol/L SA. Either calcium ionophore (A23187) or catalase inhibitor (3-amino-1,2,4-triazole; 3AT) activated PR-N gene expression. As observed for 200 μmol/L SA, the induction by A23187 and 3AT was also blocked by EGTA and reduced glutathione, respectively. A SA functional analog, 2,6-dichloroisonicotinic acid (INA), induced PR-N gene expression. However, EGTA and reduced glutathione had little or no effect on the induction by 20 μmol/L INA, same as observed for the induction by 20 μmol/L SA. Based on these data, we conclude that SA could mediate alternative signal transduction pathways leading to PR-N gene induction in tobacco cell suspension culture. SA concentration seems to play a key role in the activation of different components of signal transduction pathways. Active oxygen species elevation and external Ca2+ influx are components likely associated with 200 μmol/L SA activation mechanism. Another signal transduction pathway not associated with these two components, however, may be responsible for the effect of SA at 20 μmol/L.

KW - Calcium

KW - Glutathione

KW - Pathogenesis-related protein

KW - Salicyclic acid

KW - Signal

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