Rosuvastatin modulates the post-translational acetylome in endothelial cells

Ming Chung Lin, Chung Hsi Hsing, Fu An Li, Chien Hsing Wu, Yaw Syan Fu, Jen Kun Cheng, Bin Huang

研究成果: 雜誌貢獻文章同行評審

4 引文 斯高帕斯(Scopus)

摘要

Background: Statins are lipid-lowering drugs that can simultaneously evoke pleiotropic effects on cardioprotection, vasodilation, and diabetes prevention. Recently, statins have been reported to be able to activate the AMP-activated protein kinase, thereby up-regulating sirtuin (SIRT) that functions as non-histone deacetylases. Therefore, it is essential to investigate the post-translational acetylome that might explain the mechanism of statin-modulated pleiotropic effects. Method: Endothelial cells EAhy 926 treated with rosuvastatin were used to monitor the expression of SIRTs proteins. The protein lysates of both mock- and rosuvastatin-treated cells were further separated by two-dimensional gel electrophoresis coupled with western blotting analysis. The significantly changed acetylcontaining proteins detected by using an anti-acetyl lysine antibody were collected from another preparative gel for mass spectrometric assay to identify the acetylated site in the proteins. Results: Rosuvastatin treatment was shown to increase the SIRT1 expression when compared with SIRT2. Among 100 detected proteins with acetylated signal, 12 showed an increased level of acetylation, whereas 6 showed a decreased level of acetylation (deacetylation). The acetylated lysine (K) sites of 3 heat shock proteins, i.e., HSP47/K165, HSP70/K380, and heat shock-inducible protein/K417, were determined.We also found that beta-filamin, elongation factor, galectin and hCG22067 have 2 acetylated lysine sites in their peptide sequences. These dynamic acetylations might alter the protein's function and are thought to be important in regulating statin-mediated pleiotropic effect. Conclusions: Our study provided a feasible methodology for detecting acetylated proteins. This acetylome information may be utilized to explain, at least partially, the mechanisms of statin-derived pleiotropic effects.

原文英語
頁(從 - 到)67-73
頁數7
期刊Acta Cardiologica Sinica
30
發行號1
出版狀態已發佈 - 2014
對外發佈

ASJC Scopus subject areas

  • 心臟病學與心血管醫學

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