Rosiglitazone reduces cell invasiveness by inducing MKP-1 in human U87MG glioma cells

Hsun Jin Jan, Chin Cheng Lee, Yu Min Lin, Jing Huei Lai, Hen Wei Wei, Horng Mo Lee

研究成果: 雜誌貢獻文章同行評審

16 引文 斯高帕斯(Scopus)

摘要

We sought to investigate the molecular mechanisms by which rosiglitazone (RGZ) inhibits cell invasion in human glioma cells. In this study, we found that RGZ attenuated MMP-2 protein levels, MMP-2 gelatinolytic activity, and cell invasiveness through a PPAR-γ independent pathway. RGZ increased mitogen activated protein kinase phosphatase-1 (MKP-1) expression. The addition of triptolide (a diterpenoid triepoxide, which blocked MKP-1 induction) abolished the inhibitory effects by RGZ. Furthermore, we demonstrated that the knock down of MKP-1 by MKP-1 specific small interference RNA reversed the reduction of MMP-2 secretion, and of cell invasiveness by RGZ. In contrast, the stable expression of MKP-1 in glioma cell lines decreased MMP-2 activity and cell invasiveness. These results suggest that RGZ may mediate the inhibitory effects through MKP-1 induction. Thus, MKP-1 could be a potential target in glioma therapy.
原文英語
頁(從 - 到)141-148
頁數8
期刊Cancer Letters
277
發行號2
DOIs
出版狀態已發佈 - 5月 18 2009

ASJC Scopus subject areas

  • 癌症研究
  • 腫瘤科

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