Role of transcriptional factors Sp1, c-Rel, and c-Jun in LPS-induced C/EBPd gene expression of mouse macrophages

Y. W. Liu, C. C. Chen, J. M. Wang, W. C. Chang, Y. C. Huang, S. Y. Chung, B. K. Chen, J. J. Hung

研究成果: 雜誌貢獻文章同行評審

25 引文 斯高帕斯(Scopus)

摘要

Transcription factor C/EBPs are involved in the regulation of various cellular responses. Here, it was suggested that C/EBPd gene was activated by lipopolysaccharide (LPS) through transcription factors Sp1, c-Rel, and c-Jun. Assay of the luciferase reporter vectors containing a 5'-deletion of the C/ EBPd gene promoter indicated that a LPS-responsive element was positioned between - 345 and - 35 bp of mouse C/EBPd gene promoter. Transcription factors Sp1, c-Rel, and c-Jun bound to this region were identified using both in vivo chromatin immunoprecipitation and in vitro DNA-protein binding assays. LPS enhanced the proteins and DNA binding capacities of c-Rel and c-Jun, and the downstream Sp1 site was essential for LPS-induced C/EBPd gene. Treatment of cells with ERK/JNK/p38 inhibitors or NF-kB inhibitor inhibited the LPS-induced C/EBPd gene expression by inhibiting c-Jun, c-Rel, and p300 binding to DNA. Our findings provide a better understanding of LPS-induced C/EBPd gene expression.

原文英語
頁(從 - 到)3282-3294
頁數13
期刊Cellular and Molecular Life Sciences
64
發行號24
DOIs
出版狀態已發佈 - 12月 2007
對外發佈

ASJC Scopus subject areas

  • 細胞生物學
  • 分子生物學
  • 分子醫學
  • 藥理
  • 細胞與分子神經科學
  • 生物化學、遺傳與分子生物學 (全部)

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