Role of SUMO-Interacting Motif in Daxx SUMO Modification, Subnuclear Localization, and Repression of Sumoylated Transcription Factors

Ding Yen Lin, Yen Sung Huang, Jen Chong Jeng, Hong Yi Kuo, Che Chang Chang, Ting Ting Chao, Chun Chen Ho, Yun Ching Chen, Tong Ping Lin, Hsin I. Fang, Chih Chang Hung, Ching Shu Suen, Ming Jing Hwang, Kun Sang Chang, Gerd G. Maul, Hsiu Ming Shih

研究成果: 雜誌貢獻文章同行評審

309 引文 斯高帕斯(Scopus)

摘要

Small ubiquitin-like modifier (SUMO) modification has emerged as an important posttranslational control of protein functions. Daxx, a transcriptional corepressor, was reported to repress the transcriptional potential of several transcription factors and target to PML oncogenic domains (PODs) via SUMO-dependent interactions. The mechanism by which Daxx binds to sumoylated factors mediating transcriptional and subnuclear compartmental regulation remains unclear. Here, we define a SUMO-interacting motif (SIM) within Daxx and show it to be crucial for targeting Daxx to PODs and for transrepression of several sumoylated transcription factors, including glucocorticoid receptor (GR). In addition, the capability of Daxx SIM to bind SUMO also controls Daxx sumoylation. We further demonstrate that arsenic trioxide-induced sumoylation of PML correlates with a change of endogenous Daxx partitioning from GR-regulated gene promoter to PODs and a relief of Daxx repression on GR target gene expression. Our results provide mechanistic insights into Daxx in SUMO-dependent transcriptional control and subnuclear compartmentalization.
原文英語
頁(從 - 到)341-354
頁數14
期刊Molecular Cell
24
發行號3
DOIs
出版狀態已發佈 - 十一月 3 2006
對外發佈Yes

ASJC Scopus subject areas

  • Molecular Biology

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