Role of pirenoxine in the effects of catalin on in vitro ultravioletinduced lens protein turbidity and selenite-induced cataractogenesis in vivo

Chao Chien Hu, Jiahn Haur Liao, Kuang Yang Hsu, I. Lin Lin, Ming Hsuan Tsai, Wen Hsin Wu, Tzu Tang Wei, Yi Shiang Huang, Shih Jiuan Chiu, Hsiang Yin Chen, Shih Hsiung Wu, Tzu Hua Wu

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17 引文 斯高帕斯(Scopus)

摘要

Purpose: In this study, we investigated the biochemical pharmacology of pirenoxine (PRX) and catalin under in vitro selenite/calcium- and ultraviolet (UV)-induced lens protein turbidity challenges. The systemic effects of catalin were determined using a selenite-induced cataractogenesis rat model. Methods: In vitro cataractogenesis assay systems (including UVB/C photo-oxidation of lens crystallins, calpain-induced proteolysis, and selenite/calcium-induced turbidity of lens crystallin solutions) were used to screen the activity of PRX and catalin eye drop solutions. Turbidity was identified as the optical density measured using spectroscopy at 405 nm. We also determined the in vivo effects of catalin on cataract severity in a selenite-induced cataract rat model. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied to analyze the integrity of crystallin samples. Results: PRX at 1,000 μM significantly delayed UVC-induced turbidity formation compared to controls after 4 h of UVC exposure (p
原文英語
頁(從 - 到)1862-1870
頁數9
期刊Molecular Vision
17
出版狀態已發佈 - 2011

ASJC Scopus subject areas

  • Ophthalmology

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