Rnase a promotes proliferation of neuronal progenitor cells via an ERK-dependent pathway

Hsin Yu Liu, Chiung Ya Chen, Yun Fen Hung, Hong Ru Lin, Hsu Wen Chao, Pu Yun Shih, Chi Ning Chuang, Wei Ping Li, Tzyy Nan Huang, Yi Ping Hsueh

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

Members of the ribonuclease A (RNase A) superfamily regulate various physiological processes. RNase A, the best-studied member of the RNase A superfamily, is widely expressed in different tissues, including brains. We unexpectedly found that RNase A can trigger proliferation of neuronal progenitor cells (NPC) both in vitro and in vivo. RNase A treatment induced cell proliferation in dissociated neuronal cultures and increased cell mass in neurosphere cultures. BrdU (5-Bromo-2'-Deoxyuridine) labeling confirmed the effect of RNase A on cell proliferation. Those dividing cells were Nestin- and SOX2-positive, suggesting that RNase A triggers NPC proliferation. The proliferation inhibitor Ara-C completely suppressed the effect of RNase A on NPC counts, further supporting that RNase A increases NPC number mainly by promoting proliferation. Moreover, we found that RNase A treatment increased ERK phosphorylation and blockade of the ERK pathway inhibited the effect of RNase A on NPC proliferation. Intracerebroventricular injection of RNase A into mouse brain increased the population of 5-ethynyl-2'-deoxyuridine (EdU) or BrdU-labeled cells in the subventricular zone. Those RNase A-induced NPCs were able to migrate into other brain areas, including hippocampus, amygdala, cortex, striatum, and thalamus. In conclusion, our study shows that RNase A promotes proliferation of NPCs via an ERK-dependent pathway and further diversifies the physiological functions of the RNase A family.
原文英語
文章編號428
期刊Frontiers in Molecular Neuroscience
11
DOIs
出版狀態已發佈 - 十一月 26 2018
對外發佈Yes

指紋

Pancreatic Ribonuclease
MAP Kinase Signaling System
Ribonucleases
Stem Cells
Cell Proliferation
Bromodeoxyuridine
Brain
Cell Count
Physiological Phenomena
Nestin
Lateral Ventricles
Cytarabine
Amygdala
Thalamus

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

引用此文

Rnase a promotes proliferation of neuronal progenitor cells via an ERK-dependent pathway. / Liu, Hsin Yu; Chen, Chiung Ya; Hung, Yun Fen; Lin, Hong Ru; Chao, Hsu Wen; Shih, Pu Yun; Chuang, Chi Ning; Li, Wei Ping; Huang, Tzyy Nan; Hsueh, Yi Ping.

於: Frontiers in Molecular Neuroscience, 卷 11, 428, 26.11.2018.

研究成果: 雜誌貢獻文章

Liu, Hsin Yu ; Chen, Chiung Ya ; Hung, Yun Fen ; Lin, Hong Ru ; Chao, Hsu Wen ; Shih, Pu Yun ; Chuang, Chi Ning ; Li, Wei Ping ; Huang, Tzyy Nan ; Hsueh, Yi Ping. / Rnase a promotes proliferation of neuronal progenitor cells via an ERK-dependent pathway. 於: Frontiers in Molecular Neuroscience. 2018 ; 卷 11.
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abstract = "Members of the ribonuclease A (RNase A) superfamily regulate various physiological processes. RNase A, the best-studied member of the RNase A superfamily, is widely expressed in different tissues, including brains. We unexpectedly found that RNase A can trigger proliferation of neuronal progenitor cells (NPC) both in vitro and in vivo. RNase A treatment induced cell proliferation in dissociated neuronal cultures and increased cell mass in neurosphere cultures. BrdU (5-Bromo-2'-Deoxyuridine) labeling confirmed the effect of RNase A on cell proliferation. Those dividing cells were Nestin- and SOX2-positive, suggesting that RNase A triggers NPC proliferation. The proliferation inhibitor Ara-C completely suppressed the effect of RNase A on NPC counts, further supporting that RNase A increases NPC number mainly by promoting proliferation. Moreover, we found that RNase A treatment increased ERK phosphorylation and blockade of the ERK pathway inhibited the effect of RNase A on NPC proliferation. Intracerebroventricular injection of RNase A into mouse brain increased the population of 5-ethynyl-2'-deoxyuridine (EdU) or BrdU-labeled cells in the subventricular zone. Those RNase A-induced NPCs were able to migrate into other brain areas, including hippocampus, amygdala, cortex, striatum, and thalamus. In conclusion, our study shows that RNase A promotes proliferation of NPCs via an ERK-dependent pathway and further diversifies the physiological functions of the RNase A family.",
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author = "Liu, {Hsin Yu} and Chen, {Chiung Ya} and Hung, {Yun Fen} and Lin, {Hong Ru} and Chao, {Hsu Wen} and Shih, {Pu Yun} and Chuang, {Chi Ning} and Li, {Wei Ping} and Huang, {Tzyy Nan} and Hsueh, {Yi Ping}",
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AU - Liu, Hsin Yu

AU - Chen, Chiung Ya

AU - Hung, Yun Fen

AU - Lin, Hong Ru

AU - Chao, Hsu Wen

AU - Shih, Pu Yun

AU - Chuang, Chi Ning

AU - Li, Wei Ping

AU - Huang, Tzyy Nan

AU - Hsueh, Yi Ping

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N2 - Members of the ribonuclease A (RNase A) superfamily regulate various physiological processes. RNase A, the best-studied member of the RNase A superfamily, is widely expressed in different tissues, including brains. We unexpectedly found that RNase A can trigger proliferation of neuronal progenitor cells (NPC) both in vitro and in vivo. RNase A treatment induced cell proliferation in dissociated neuronal cultures and increased cell mass in neurosphere cultures. BrdU (5-Bromo-2'-Deoxyuridine) labeling confirmed the effect of RNase A on cell proliferation. Those dividing cells were Nestin- and SOX2-positive, suggesting that RNase A triggers NPC proliferation. The proliferation inhibitor Ara-C completely suppressed the effect of RNase A on NPC counts, further supporting that RNase A increases NPC number mainly by promoting proliferation. Moreover, we found that RNase A treatment increased ERK phosphorylation and blockade of the ERK pathway inhibited the effect of RNase A on NPC proliferation. Intracerebroventricular injection of RNase A into mouse brain increased the population of 5-ethynyl-2'-deoxyuridine (EdU) or BrdU-labeled cells in the subventricular zone. Those RNase A-induced NPCs were able to migrate into other brain areas, including hippocampus, amygdala, cortex, striatum, and thalamus. In conclusion, our study shows that RNase A promotes proliferation of NPCs via an ERK-dependent pathway and further diversifies the physiological functions of the RNase A family.

AB - Members of the ribonuclease A (RNase A) superfamily regulate various physiological processes. RNase A, the best-studied member of the RNase A superfamily, is widely expressed in different tissues, including brains. We unexpectedly found that RNase A can trigger proliferation of neuronal progenitor cells (NPC) both in vitro and in vivo. RNase A treatment induced cell proliferation in dissociated neuronal cultures and increased cell mass in neurosphere cultures. BrdU (5-Bromo-2'-Deoxyuridine) labeling confirmed the effect of RNase A on cell proliferation. Those dividing cells were Nestin- and SOX2-positive, suggesting that RNase A triggers NPC proliferation. The proliferation inhibitor Ara-C completely suppressed the effect of RNase A on NPC counts, further supporting that RNase A increases NPC number mainly by promoting proliferation. Moreover, we found that RNase A treatment increased ERK phosphorylation and blockade of the ERK pathway inhibited the effect of RNase A on NPC proliferation. Intracerebroventricular injection of RNase A into mouse brain increased the population of 5-ethynyl-2'-deoxyuridine (EdU) or BrdU-labeled cells in the subventricular zone. Those RNase A-induced NPCs were able to migrate into other brain areas, including hippocampus, amygdala, cortex, striatum, and thalamus. In conclusion, our study shows that RNase A promotes proliferation of NPCs via an ERK-dependent pathway and further diversifies the physiological functions of the RNase A family.

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