Rice husk silica liquid protects pancreatic β cells from streptozotocin-induced oxidative damage

Hsin Yuan Chen, Yi Fen Chiang, Kai Lee Wang, Tsui Chin Huang, Mohamed Ali, Tzong Ming Shieh, Hsin Yi Chang, Yong Han Hong, Shih Min Hsia

研究成果: 雜誌貢獻文章同行評審

摘要

Type 2 diabetes mellitus is a complex multifactorial disease characterized by insulin resistance and dysfunction of pancreatic β-cells. Rice husk silica liquid (RHSL) is derived from rice husks and has not been explored in diabetes mellitus until now. Previous studies showed that rice husk is enriched with silica, and its silica nanoparticles are higher more biocompatible. To investi-gate the potential protective role of RHSL on pancreatic β cells, we utilized RIN-m5F pancreatic β cells and explored RHSL effect after streptozotocin (STZ)-stimulation. The recovery effects of RHSL were evaluated using flow cytometry, Western blotting, and immunofluorescence analysis. Results of our study showed that RHSL reversed the cell viability, insulin secretion, reactive oxygen species (ROS) production, and the change of mitochondria membrane potential (ΔΨm) in STZ-treated RIN-m5F cells. Moreover, the expression of phospho-receptor-interacting protein 3 (p-RIP3) and cleaved-poly (ADP-ribose) polymerase (PARP), phospho-mammalian target of rapamycin (p-mTOR), and sequestosome-1 (p62/SQSTM1) were significantly decreased, while the transition of light chain (LC)3-I to LC3-II was markedly increased after RHSL treatment in STZ-induced RIN-m5F cells. Interestingly, using autophagy inhibitors such as 3-methyladenine (3-MA) and chloro-quine (CQ) both showed an increase in cleaved-PARP protein level, indicating apoptosis induction. Taken together, this study demonstrated that RHSL induced autophagy and alleviated STZ-in-duced ROS-mediated apoptosis in RIN-m5F cells.
原文英語
文章編號1080
期刊Antioxidants
10
發行號7
DOIs
出版狀態已發佈 - 7月 2021

ASJC Scopus subject areas

  • 生物化學
  • 生理學
  • 分子生物學
  • 臨床生物化學
  • 細胞生物學

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