Review: Macro-encapsulation of islets in polyvinyl alcohol hydrogel

Shoichiro Sumi, Goichi Yanai, Meirigeng Qi, Naoaki Sakata, Zhi Qi, Kaichiang Yang, Yasumasa Shirouzu, Akihiko Hiura, Yuangjun Gu, Kazutomo Inoue

研究成果: 雜誌貢獻回顧型文獻

5 引文 (Scopus)

摘要

Diabetes mellitus (DM) can be cured by adequate insulin secretion from a relatively small volume of cells. Cell encapsulation enables allo- and even xeno-geneic cell therapy without immunosuppression. Micro-encapsulated islets used in recent clinical trials are not fully retrievable after transplantation. This paper summerizes the development of retrievable and theoretically replaceable macro-encapsulated islets using polyvinyl alcohol (PVA) hydrogel. An aqueous solution of PVA becomes a gel through micro crystallization by freezing and thawing. Utilizing this feature, PVA-macro-encapsulated islets (PVA-MEIs) were developed. Rat islets suspended in Euro-Collins solution containing 3% PVA were encapsulated in a mesh-reinforced PVA hydrogel sheet by freezing and thawing. The feasibility of PVA-MEIs for DM therapy was tested in vitro and in vivo. PVA-MEIs showed glucose-responsive insulin secretion in vitro even after 14-day culture. Rat PVA-MEIs cultured in media containing fresh human plasma showed no morphological changes and maintained insulin content. Intra-peritoneal transplantation of PVA-MEIs containing 750 rat islets ameliorated hyperglycemia in streptozotocin (STZ)-induced diabetic mice to nearly normal levels for up to 30 days with a consistent increase in body weight. Transplantation of PVA-MEIs also prevented metabolic and renal disorders in STZ-induced diabetic mice. PVA-MEIs cryo-preserved for 1, 7, and 30 days showed similar function in vitro and corrected hyperglycemia after intra-peritoneal transplantation in STZ-induced diabetic mice. Intra-peritoneal transplantation of PVA-MEIs containing iso- or allo-geneic islets (approx. 2,000 islets) ameliorated hyperglycemia in STZ-induced diabetic rats in a similar manner for almost half a year although the efficacy gradually decreased with time. Transplantation of PVA-MEIs ameliorated hyperglycemia in diabetic mice and rats without immunosupression. Retrievable and theoretically replaceable PVA-MEIs that can secure cell entrapment can mitigate the potential risks associated with xeno-geneic cells and cells made from undifferentiated cells. Therefore, PVA-MEIs are a promising modality for future DM therapy.
原文英語
頁(從 - 到)204-210
頁數7
期刊Journal of Medical and Biological Engineering
34
發行號3
DOIs
出版狀態已發佈 - 2014

指紋

Polyvinyl Alcohol
Polyvinyl alcohols
Encapsulation
Hydrogels
Macros
Transplantation
Streptozocin
Hyperglycemia
Rats
Insulin
Medical problems
Diabetes Mellitus
Thawing
polyvinyl alcohol hydrogel
Freezing
Plasma (human)
Cell- and Tissue-Based Therapy
Crystallization
Cell Size
Immunosuppression

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)

引用此文

Review : Macro-encapsulation of islets in polyvinyl alcohol hydrogel. / Sumi, Shoichiro; Yanai, Goichi; Qi, Meirigeng; Sakata, Naoaki; Qi, Zhi; Yang, Kaichiang; Shirouzu, Yasumasa; Hiura, Akihiko; Gu, Yuangjun; Inoue, Kazutomo.

於: Journal of Medical and Biological Engineering, 卷 34, 編號 3, 2014, p. 204-210.

研究成果: 雜誌貢獻回顧型文獻

Sumi, S, Yanai, G, Qi, M, Sakata, N, Qi, Z, Yang, K, Shirouzu, Y, Hiura, A, Gu, Y & Inoue, K 2014, 'Review: Macro-encapsulation of islets in polyvinyl alcohol hydrogel', Journal of Medical and Biological Engineering, 卷 34, 編號 3, 頁 204-210. https://doi.org/10.5405/jmbe.1579
Sumi, Shoichiro ; Yanai, Goichi ; Qi, Meirigeng ; Sakata, Naoaki ; Qi, Zhi ; Yang, Kaichiang ; Shirouzu, Yasumasa ; Hiura, Akihiko ; Gu, Yuangjun ; Inoue, Kazutomo. / Review : Macro-encapsulation of islets in polyvinyl alcohol hydrogel. 於: Journal of Medical and Biological Engineering. 2014 ; 卷 34, 編號 3. 頁 204-210.
@article{f23207d0dcf5474484dea05396a93d06,
title = "Review: Macro-encapsulation of islets in polyvinyl alcohol hydrogel",
abstract = "Diabetes mellitus (DM) can be cured by adequate insulin secretion from a relatively small volume of cells. Cell encapsulation enables allo- and even xeno-geneic cell therapy without immunosuppression. Micro-encapsulated islets used in recent clinical trials are not fully retrievable after transplantation. This paper summerizes the development of retrievable and theoretically replaceable macro-encapsulated islets using polyvinyl alcohol (PVA) hydrogel. An aqueous solution of PVA becomes a gel through micro crystallization by freezing and thawing. Utilizing this feature, PVA-macro-encapsulated islets (PVA-MEIs) were developed. Rat islets suspended in Euro-Collins solution containing 3{\%} PVA were encapsulated in a mesh-reinforced PVA hydrogel sheet by freezing and thawing. The feasibility of PVA-MEIs for DM therapy was tested in vitro and in vivo. PVA-MEIs showed glucose-responsive insulin secretion in vitro even after 14-day culture. Rat PVA-MEIs cultured in media containing fresh human plasma showed no morphological changes and maintained insulin content. Intra-peritoneal transplantation of PVA-MEIs containing 750 rat islets ameliorated hyperglycemia in streptozotocin (STZ)-induced diabetic mice to nearly normal levels for up to 30 days with a consistent increase in body weight. Transplantation of PVA-MEIs also prevented metabolic and renal disorders in STZ-induced diabetic mice. PVA-MEIs cryo-preserved for 1, 7, and 30 days showed similar function in vitro and corrected hyperglycemia after intra-peritoneal transplantation in STZ-induced diabetic mice. Intra-peritoneal transplantation of PVA-MEIs containing iso- or allo-geneic islets (approx. 2,000 islets) ameliorated hyperglycemia in STZ-induced diabetic rats in a similar manner for almost half a year although the efficacy gradually decreased with time. Transplantation of PVA-MEIs ameliorated hyperglycemia in diabetic mice and rats without immunosupression. Retrievable and theoretically replaceable PVA-MEIs that can secure cell entrapment can mitigate the potential risks associated with xeno-geneic cells and cells made from undifferentiated cells. Therefore, PVA-MEIs are a promising modality for future DM therapy.",
keywords = "Bio-artificial pancreas, Diabetes mellitus(DM), Macro-encapsulated islets (MEIs), Polyvinyl alcohol (PVA)",
author = "Shoichiro Sumi and Goichi Yanai and Meirigeng Qi and Naoaki Sakata and Zhi Qi and Kaichiang Yang and Yasumasa Shirouzu and Akihiko Hiura and Yuangjun Gu and Kazutomo Inoue",
year = "2014",
doi = "10.5405/jmbe.1579",
language = "English",
volume = "34",
pages = "204--210",
journal = "Journal of Medical and Biological Engineering",
issn = "1609-0985",
publisher = "中華民國生物醫學工程學會",
number = "3",

}

TY - JOUR

T1 - Review

T2 - Macro-encapsulation of islets in polyvinyl alcohol hydrogel

AU - Sumi, Shoichiro

AU - Yanai, Goichi

AU - Qi, Meirigeng

AU - Sakata, Naoaki

AU - Qi, Zhi

AU - Yang, Kaichiang

AU - Shirouzu, Yasumasa

AU - Hiura, Akihiko

AU - Gu, Yuangjun

AU - Inoue, Kazutomo

PY - 2014

Y1 - 2014

N2 - Diabetes mellitus (DM) can be cured by adequate insulin secretion from a relatively small volume of cells. Cell encapsulation enables allo- and even xeno-geneic cell therapy without immunosuppression. Micro-encapsulated islets used in recent clinical trials are not fully retrievable after transplantation. This paper summerizes the development of retrievable and theoretically replaceable macro-encapsulated islets using polyvinyl alcohol (PVA) hydrogel. An aqueous solution of PVA becomes a gel through micro crystallization by freezing and thawing. Utilizing this feature, PVA-macro-encapsulated islets (PVA-MEIs) were developed. Rat islets suspended in Euro-Collins solution containing 3% PVA were encapsulated in a mesh-reinforced PVA hydrogel sheet by freezing and thawing. The feasibility of PVA-MEIs for DM therapy was tested in vitro and in vivo. PVA-MEIs showed glucose-responsive insulin secretion in vitro even after 14-day culture. Rat PVA-MEIs cultured in media containing fresh human plasma showed no morphological changes and maintained insulin content. Intra-peritoneal transplantation of PVA-MEIs containing 750 rat islets ameliorated hyperglycemia in streptozotocin (STZ)-induced diabetic mice to nearly normal levels for up to 30 days with a consistent increase in body weight. Transplantation of PVA-MEIs also prevented metabolic and renal disorders in STZ-induced diabetic mice. PVA-MEIs cryo-preserved for 1, 7, and 30 days showed similar function in vitro and corrected hyperglycemia after intra-peritoneal transplantation in STZ-induced diabetic mice. Intra-peritoneal transplantation of PVA-MEIs containing iso- or allo-geneic islets (approx. 2,000 islets) ameliorated hyperglycemia in STZ-induced diabetic rats in a similar manner for almost half a year although the efficacy gradually decreased with time. Transplantation of PVA-MEIs ameliorated hyperglycemia in diabetic mice and rats without immunosupression. Retrievable and theoretically replaceable PVA-MEIs that can secure cell entrapment can mitigate the potential risks associated with xeno-geneic cells and cells made from undifferentiated cells. Therefore, PVA-MEIs are a promising modality for future DM therapy.

AB - Diabetes mellitus (DM) can be cured by adequate insulin secretion from a relatively small volume of cells. Cell encapsulation enables allo- and even xeno-geneic cell therapy without immunosuppression. Micro-encapsulated islets used in recent clinical trials are not fully retrievable after transplantation. This paper summerizes the development of retrievable and theoretically replaceable macro-encapsulated islets using polyvinyl alcohol (PVA) hydrogel. An aqueous solution of PVA becomes a gel through micro crystallization by freezing and thawing. Utilizing this feature, PVA-macro-encapsulated islets (PVA-MEIs) were developed. Rat islets suspended in Euro-Collins solution containing 3% PVA were encapsulated in a mesh-reinforced PVA hydrogel sheet by freezing and thawing. The feasibility of PVA-MEIs for DM therapy was tested in vitro and in vivo. PVA-MEIs showed glucose-responsive insulin secretion in vitro even after 14-day culture. Rat PVA-MEIs cultured in media containing fresh human plasma showed no morphological changes and maintained insulin content. Intra-peritoneal transplantation of PVA-MEIs containing 750 rat islets ameliorated hyperglycemia in streptozotocin (STZ)-induced diabetic mice to nearly normal levels for up to 30 days with a consistent increase in body weight. Transplantation of PVA-MEIs also prevented metabolic and renal disorders in STZ-induced diabetic mice. PVA-MEIs cryo-preserved for 1, 7, and 30 days showed similar function in vitro and corrected hyperglycemia after intra-peritoneal transplantation in STZ-induced diabetic mice. Intra-peritoneal transplantation of PVA-MEIs containing iso- or allo-geneic islets (approx. 2,000 islets) ameliorated hyperglycemia in STZ-induced diabetic rats in a similar manner for almost half a year although the efficacy gradually decreased with time. Transplantation of PVA-MEIs ameliorated hyperglycemia in diabetic mice and rats without immunosupression. Retrievable and theoretically replaceable PVA-MEIs that can secure cell entrapment can mitigate the potential risks associated with xeno-geneic cells and cells made from undifferentiated cells. Therefore, PVA-MEIs are a promising modality for future DM therapy.

KW - Bio-artificial pancreas

KW - Diabetes mellitus(DM)

KW - Macro-encapsulated islets (MEIs)

KW - Polyvinyl alcohol (PVA)

UR - http://www.scopus.com/inward/record.url?scp=84903781346&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903781346&partnerID=8YFLogxK

U2 - 10.5405/jmbe.1579

DO - 10.5405/jmbe.1579

M3 - Review article

AN - SCOPUS:84903781346

VL - 34

SP - 204

EP - 210

JO - Journal of Medical and Biological Engineering

JF - Journal of Medical and Biological Engineering

SN - 1609-0985

IS - 3

ER -