Resistin contributes to neointimal formation via oxidative stress after vascular injury

Kou-Gi Shyu, Li Ming Lien, Bao Wei Wang, Peiliang Kuan, Hang Chang

研究成果: 雜誌貢獻文章

15 引文 (Scopus)

摘要

Resistin may play a major potential role in vascular remodelling and may contribute to atherogenesis. However, the role of VSMC (vascular smooth muscle cell)-derived resistin in neointimal formation is not well understood. We hypothesize that endogenous resistin derived from VSMCs may contribute to neointimal formation after vascular injury. VSMCs from thoracic aorta of adult Wistar rats were cultured. The carotid artery from adult Wistar rats was injured by balloon catheter. Resistin significantly increased migration and proliferation of VSMCs. Resistin siRNA (small interfering RNA) and resistin antibody significantly inhibited migration and proliferation of VSMCs induced by conditioned medium from stretched VSMCs. Resistin protein and mRNA expression significantly increased at 14 days after carotid injury. Resistin siRNA and NAC (N-acetylcysteine) significantly reduced resistin protein and mRNA expression induced by balloon injury. Carotid artery injury increased ROS (reactive oxygen species) production. Treatment with NAC and resistin siRNA decreased ROS production. The neointimal area was significantly increased after carotid injury and was significantly reduced by resistin siRNA and NAC. In conclusion, resistin increases migration and proliferation of VSMCs, and expression of resistin in carotid artery significantly increases after injury. Resistin siRNA attenuates neointimal formation after carotid injury partly through an antioxidative mechanism. Resistin may play a pivotal role in the pathogenesis of neointimal thickening after mechanical injury.
原文英語
頁(從 - 到)121-129
頁數9
期刊Clinical Science
120
發行號3
DOIs
出版狀態已發佈 - 二月 2011

指紋

Resistin
Vascular System Injuries
Oxidative Stress
Small Interfering RNA
Wounds and Injuries
Acetylcysteine
Carotid Arteries
Wistar Rats
Reactive Oxygen Species
Carotid Artery Injuries
Messenger RNA
Conditioned Culture Medium

ASJC Scopus subject areas

  • Medicine(all)

引用此文

Resistin contributes to neointimal formation via oxidative stress after vascular injury. / Shyu, Kou-Gi; Lien, Li Ming; Wang, Bao Wei; Kuan, Peiliang; Chang, Hang.

於: Clinical Science, 卷 120, 編號 3, 02.2011, p. 121-129.

研究成果: 雜誌貢獻文章

Shyu, Kou-Gi ; Lien, Li Ming ; Wang, Bao Wei ; Kuan, Peiliang ; Chang, Hang. / Resistin contributes to neointimal formation via oxidative stress after vascular injury. 於: Clinical Science. 2011 ; 卷 120, 編號 3. 頁 121-129.
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