Repeated-batch lactic acid fermentation using a novel bacterial immobilization technique based on a microtube array membrane

Chien Chung Chen, Chuan Chi Lan, Chorng Liang Pan, Mei Ying Huang, Chee Ho Chew, Chin Chieh Hung, Po Hsuan Chen, Hong Ting Victor Lin

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)

摘要

Lactic acid has received considerable attention for its various applications. Although lactic acid is usually produced by batch fermentation using free microbes, repeated-batch fermentation using immobilized cells could offer several advantages over this method. In the present study, a novel bacteria immobilization technique using a renewable poly-L-lactic acid (PLLA) microtube array membrane (MTAM) was thoroughly evaluated in terms of its suitability for lactic acid fermentation. A bacteria encapsulation efficiency of 85–90% was obtained using a siphon approach, and bacteria in MTAMs with greater porosity showed greater lactic acid productivity. MTAM-immobilized Lactobacillus acidophilus were evaluated in eight cycles of repeated-batch lactic acid fermentation of commercial medium MRS (4% glucose) and red seaweed Gracilaria hydrolysate. The MTAM exhibited physical stability during lactic acid fermentation and improved glucose consumption and lactic acid production were achieved by immobilized cells. The immobilized bacteria showed a maximum CLA of 37.39 g/L, r PLA of 0.79 g/L·h, and YL/S of 0.94 g/g in MRS fermentation, and a maximum CLA of 27.76 g/L, r PLA of 0.58 g/L·h, and YL/S of 0.92 g/g in seaweed hydrolysate fermentation. Our data indicate that PLLA-MTAM is a novel, promising immobilization technology for lactic acid fermentation.

原文英語
頁(從 - 到)25-32
頁數8
期刊Process Biochemistry
87
DOIs
出版狀態已發佈 - 十二月 2019

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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