Reparixin attenuates neuronal injury in experimental Klebsiella pneumoniae meningoencephalitis through dual effects on neuroprotection and neuroinflammation

Chien-Tsai Chiu, Li-Li Wen, Hsin-Ping Pao, Ling-Yu Yang, Ya-Ni Huang, Jia-Yi Wang

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Aims: Bacterial meningitis causes high mortality and brain damage. The host immune response is associated with brain injury. Chemokine (C-X-C motif) (CXC) chemokines are neutrophil chemoattractants. This study focused on the beneficial effects of intracerebroventricular administration of reparixin, an inhibitor of chemokine (C-X-C motif) receptor (CXCR)1/2, to rats at 2h following experimental Klebsiella pneumoniae meningoencephalitis. Methods: We used a previously established meningoencephalitis animal model in which Sprague-Dawley rats were infected by K.pneumoniae. Sham and infected animals were treated with vehicle or reparixin and sacrificed at various time points. Leukocyte infiltration into cerebrospinal fluid (CSF) and brain as well as gene and protein expression of chemokines and receptors, and neuronal apoptosis were examined. Primary cultures of neuron/glia were infected with K.pneumoniae as an in vitro model of meningoencephalitis. Results: Levels of chemokine (C-X-C motif) ligand (CXCL)2 in CSF time-dependently increased markedly as early as 2h, and peaked at 8h following infection and were much higher than those in serum collected simultaneously. Reparixin significantly reduced leukocyte infiltration into CSF and brain tissues, clinical illness, and brain cell apoptosis at 24h. Reparixin reduced the elevated CSF concentrations of chemokines [CXCL1, CXCL2, chemokine (C-C motif) ligand (CCL)2 and CCL5] and proinflammatory cytokines. Reparixin also reduced the expression of mRNA of various chemokines, chemokine receptors and proinflammatory cytokines in infected brain tissues. Using primary cultures that are devoid of leukocytes, we further observed that reparixin attenuated the neuronal, but not microglial cell death after infection. Conclusions: Reparixin not only reduces amplified inflammation, but also provides direct neuroprotective effects in K.pneumoniae meningoencephalitis.
原文英語
頁(從 - 到)326-343
頁數18
期刊Neuropathology and Applied Neurobiology
42
發行號4
DOIs
出版狀態已發佈 - 六月 1 2016

ASJC Scopus subject areas

  • 神經病學(臨床)
  • 病理學與法醫學
  • 神經內科
  • 組織學
  • 生理學(醫學)

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