Renoprotective Effect of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Immunodeficient Mice Suffering from Acute Kidney Injury

Te Chao Fang, Cheng Yoong Pang, Sheng Chun Chiu, Dah Ching Ding, Rong Kung Tsai

研究成果: 雜誌貢獻文章

21 引文 斯高帕斯(Scopus)

摘要

It is unknown whether human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) can improve the renal function of patients suffering from acute kidney injury. Moreover, before beginning clinical trials, it is necessary to investigate this renoprotective effect of hUC-MSCs in a xenogeneic model of acute kidney injury. However, no previous studies have examined the application of hUC-MSCs to immunodeficient mice suffering from acute kidney injury. The objectives of this study were to examine whether hUC-MSCs could improve renal function in nonobese diabetic-severe combined immune deficiency (NOD-SCID) mice suffering from acute kidney injury, and to investigate the mechanism(s) for hUC-MSCs to improve renal function in this xenogeneic model. Early (3 hr) and late (12 hr) administrations of hUC-MSCs (106 cells) were performed via the external jugular vein into NOD-SCID mice suffering from either folic acid (FA) (250 mg/kg body weight) or vehicle. The results showed that early administration of hUC-MSCs improved the renal function of NOD-SCID mice suffering from FA-induced acute kidney injury, as evidenced by decreased serum urea nitrogen and serum creatinine levels, as well as a reduced tubular injury score. The beneficial effects of hUC-MSCs were through reducing apoptosis and promoting proliferation of renal tubular cells. These benefits were independent of inflammatory cytokine effects and transdifferentiation. Furthermore, this study is the first one to show that the reduced apoptosis of renal tubular cells by hUC-MSCs in this xenogeneic model is mediated through the mitochondrial pathway, and through the increase of Akt phosphorylation.

原文英語
文章編號e46504
期刊PLoS One
7
發行號9
DOIs
出版狀態已發佈 - 九月 27 2012
對外發佈Yes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

指紋 深入研究「Renoprotective Effect of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Immunodeficient Mice Suffering from Acute Kidney Injury」主題。共同形成了獨特的指紋。

  • 引用此