Relaxation of isolated guinea pig trachea by genistein via inhibition of phosphodiesterase

Ching Chi Lin, Junn Lain Chen, Wun Chang Ko

研究成果: 雜誌貢獻文章同行評審

8 引文 斯高帕斯(Scopus)

摘要

We investigated the mechanisms of the relaxant action of genistein, an isoflavone, phytoestrogen and non-specific protein tyrosine kinase inhibitor. Changes in tension of guinea pig tracheal segments were isometrically recorded on a polygraph. Genistein concentration-dependently relaxed histamine (30 μM)-, carbachol (0.2 μM)-, KC1 (30 mM)- and leukotriene D4 (10 nM)-induced precontractions and inhibited cumulative histamine- and carbachol-induced contractions in a non-competitive manner. Genistein also concentration-dependently and non-competitively inhibited the cumulative, Ca2+-induced contractions in the depolarized (K+, 60 mM) trachealis. The remaining nifedipine (10 μM)-induced tension of the histamine (30 μM)-induced precontraction was further relaxed by genistein, suggesting that regardless of whether voltage-dependent calcium channels are blocked genistein may have other mechanisms of relaxant action. These other mechanisms of the relaxant effect of genistein appeared to be epithelium-independent and were not affected by the presence of propranolol (1 μM), 2′5′- dideoxyadenosine (10 ′M), methylene blue (25 μM), glibenclamide (10 μM), Nω-nitro-L-arginine (20 ωM) or α-chymotrypsin (1 U/mL), suggesting that the mechanisms are unrelated to activation of the β-adrenoceptor, of adenylate cyclase, of guanylate cyclase, of adenosine triphosphatesensitive potassium channel opening, of nitric oxide formation or of neuropeptide release, respectively. However, genistein (17.5-35 μM) produced parallel, leftward shifts in the concentration-response curves of forskolin and nitroprusside and significantly increased the pD2 values of these two agonists. Both genistein and 3-isobutyl-1-methylxanthine at various concentrations (10-300 μM) concentration-dependently and significantly inhibited cAMP- and cGMP-phosphodiesterase (PDE) activities of the trachealis. The -log IC50 values of genistein were estimated to be 4.28 and 4.17, respectively. The above results reveal that the mechanisms of the relaxant action of genistein may be due to its non-selective inhibition of both PDE activities.
原文英語
頁(從 - 到)323-329
頁數7
期刊Planta Medica
73
發行號4
DOIs
出版狀態已發佈 - 4月 2007

ASJC Scopus subject areas

  • 植物科學
  • 藥物發現
  • 有機化學
  • 藥理

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