Regulatory role of DC-derived osteopontin in systemic allergen sensitization

Masatsugu Kurokawa, Satoshi Konno, Ayumu Takahashi, Beverly Plunkett, Susan R. Rittling, Yutaka Matsui, Shigeyuki Kon, Junko Morimoto, Toshimitsu Uede, Satoshi Matsukura, Fumio Kokubu, Mitsuru Adachi, Masaharu Nishimura, Shau Ku Huang

研究成果: 雜誌貢獻文章同行評審

16 引文 斯高帕斯(Scopus)

摘要

Osteopontin (OPN) is a secreted phosphoglycoprotein with a wide range of functions, and is involved in various pathophysiological conditions. However, the role of OPN in IgE and Th2-associated allergic responses remains incompletely defined. The aim of this study was to elucidate the role of OPN in systemic allergen sensitization in mice. When compared with OPN+/+ mice, significantly increased levels of OVA-induced IgE were found in OPN -/- mice. OPN-/- DC demonstrated an increased capacity to enhance Th2 cytokine production in CD4+ T cells from sensitized OPN+/+ mice. Furthermore, significantly reduced levels of IL-12p70 expression were seen in LPS-stimulated OPN-/- DC as compared with the WT DC, and the reduction was reversible by the addition of recombinant OPN (rOPN). rOPN was able to suppress OVA-induced IL-13 production in the cultures of CD4 and OPN-/- DC, but this inhibitory activity was neutralized by the addition of anti-IL-12 Ab. In addition, administration of rOPN in vivo suppressed OVA-specific IgE production; however, this suppressive effect was abrogated in IL-12-deficient mice. These results indicate that DC-derived OPN plays a regulatory role in the development of systemic allergen sensitization, which is mediated, at least in part, through the production of endogenous IL-12.

原文英語
頁(從 - 到)3323-3330
頁數8
期刊European Journal of Immunology
39
發行號12
DOIs
出版狀態已發佈 - 十二月 1 2009
對外發佈

ASJC Scopus subject areas

  • 免疫學和過敏
  • 免疫學

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