Regulation of topoisomerase II expression during the VM-26 induced differentiation of IW32 murine erythroleukemia cells

Sai Koong Tan, Shye Jay Tang, Mei Chih Wang, Jaulang Hwang, Fung Fang Wang

研究成果: 雜誌貢獻文章同行評審

3 引文 斯高帕斯(Scopus)

摘要

The effect of VM-26, a topoisomerase II targeting drug, on IW32 murine erythroleukemia cells was investigated. The VM-26 induced IW32 cells to differentiate at a non-toxic but cytostatic concentration (0.01 μg/ml). More than 40% of the cells were induced to synthesize hemoglobin, and cells were arrested in G2/M phase of the cell cycle. Levels of β-globin mRNA also increased significantly. Cells became committed to erythroid maturation after 16 h of continuous drug exposure. Replacement with fresh VM-26 after 48 h of drug treatment further increased the hemoglobin containing cells to greater than 80%. Unlike other drug induced erythroleukemia cell differentiation, c-myc mRNA expression was not affected by VM-26. Inhibition of topoisomerase II activity was observed during the first 12 h of VM-26 treatment; however, elevated enzyme activity was found thereafter. Northern blot analysis showed significant increase in the expression of topoisomerase IIα mRNA at 12 and 24 h after VM-26 addition. These findings indicate that VM-26 inhibited the activity of topoisomerase II and promoted the committed differentiation of IW32 cells along the erythroid pathway. In addition, a parallel increase in mRNA and activity levels of topoisomerase II in differentiated cells suggests that regulation of the enzyme expression occurred in the VM-26 induced erythroid maturation.

原文英語
頁(從 - 到)249-257
頁數9
期刊Leukemia Research
20
發行號3
DOIs
出版狀態已發佈 - 3月 1996
對外發佈

ASJC Scopus subject areas

  • 血液學
  • 腫瘤科
  • 癌症研究

指紋

深入研究「Regulation of topoisomerase II expression during the VM-26 induced differentiation of IW32 murine erythroleukemia cells」主題。共同形成了獨特的指紋。

引用此