Regulation of Th2 cytokine genes by p38 MAPK-mediated phosphorylation of GATA-3

Kittipong Maneechotesuwan, Yao Xin, Kazuhiro Ito, Elen Jazrawi, Kang Yun Lee, Omar S. Usmani, Peter J. Barnes, Ian M. Adcock

研究成果: 雜誌貢獻文章同行評審

124 引文 斯高帕斯(Scopus)

摘要

GATA-3 plays a critical role in allergic diseases by regulating the release of cytokines from Th2 lymphocytes. However, the molecular mechanisms involved in the regulation of GATA-3 in human T lymphocytes are not yet understood. Using small interfering RNA to knock down GATA-3, we have demonstrated its critical role in regulating IL-4, IL-5, and IL-13 release from a human T cell line. Specific stimulation of T lymphocytes by costimulation of CD3 and CD28 to mimic activation by APCs induces translocation of GATA-3 from the cytoplasm to the nucleus, with binding to the promoter region of Th2 cytokine genes, as determined by chromatin immunoprecipitation. GATA-3 nuclear translocation is dependent on its phosphorylation on serine residues by p38 MAPK, which facilitates interaction with the nuclear transporter protein importin-α. This provides a means whereby allergen exposure leads to the expression of Th2 cytokines, and this novel mechanism may provide new approaches to treating allergic diseases.

原文英語
頁(從 - 到)2491-2498
頁數8
期刊Journal of Immunology
178
發行號4
出版狀態已發佈 - 2月 15 2007
對外發佈

ASJC Scopus subject areas

  • 免疫學

指紋

深入研究「Regulation of Th2 cytokine genes by p38 MAPK-mediated phosphorylation of GATA-3」主題。共同形成了獨特的指紋。

引用此